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PMS2

ANTIBODY INFORMATION
 
 

Antibody HPA044400

  

Antibody HPA066490

  

Antibody HPA070310

  

Antibody CAB010235

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA044400   HPA066490   HPA070310   sc-618  

Host species

 Rabbit   Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

        

Released in version

 12   16   16   3  

References

        

Proper citation

 Atlas Antibodies Cat#HPA044400, RRID:AB_2678929   Atlas Antibodies Cat#HPA066490, RRID:AB_2685677   Atlas Antibodies Cat#HPA070310, RRID:AB_2686250   Santa Cruz Biotechnology Cat#sc-618, RRID:AB_2167281  

VALIDATION SUMMARY
 
 

Antibody HPA044400

  

Antibody HPA066490

  

Antibody HPA070310

  

Antibody CAB010235

  
Immunocytochemistry

Supported   Supported   Supported   Supported  
Immunohistochemistry

      Approved  
Western blot

Supported   Uncertain   Uncertain   Supported  
Protein array

Supported   Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA044400

  

Antibody HPA066490

  

Antibody HPA070310

  

Antibody CAB010235

  

Standard validation

 Supported   Supported   Supported   Supported  

       

Figure description

 Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & microtubules.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane.   Immunofluorescent staining of human cell line HaCaT shows localization to nucleoplasm & cytoplasmic bodies.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.  

Antibody dilution

 1:68   1:65   1:200   1:13  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA044400

  

Antibody HPA066490

  

Antibody HPA070310

  

Antibody CAB010235

  

Standard validation

       Approved  

       

Figure description

       Immunohistochemical staining of human cerebellum shows strong nuclear positivity in purkinje cells.  

Expression

       RNA: detected in 36 tissues
Protein: detected in 62 cell types		  

Retrieval

       HIER pH6  

Antibody dilution

       1:50  

Literature conformity

       Partly consistent with gene/protein characterization data.  

RNA consistency

       Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA044400

  

Antibody HPA066490

  

Antibody HPA070310

  

Antibody CAB010235

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

       

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		       Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 95.8, 84.7, 51.3   95.8, 84.7   95.8, 84.7   95.8, 84.7, 51.3  

Antibody dilution

 1:250   1:220   1:1040   1:500  

PROTEIN ARRAY
 
 

Antibody HPA044400

  

Antibody HPA066490

  

Antibody HPA070310

  

Antibody CAB010235

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:500   1:12450    

ANTIGEN INFORMATION
 
 

Antibody HPA044400

  

Antibody HPA066490

  

Antibody HPA070310

  

Antibody CAB010235

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 98   59   59    

Antigen sequence

 
AKLISLPTSKNWTFGPQDVDELIFMLSDSPGVMCRPSRVKQMFASRACRK
SVMIGTALNTSEMKKLITHMGEMDHPWNCPHGRPTMRHIANLGVISQN
  
AISDKGVLRPQKEAVSSSQGPSDPTDRAEVEKDSGHGSTSVDSEGFSIPD
TGSHCSSEC
  
MHAADLEKPMVEKQDQSPSLRTGEEKKDVSISRLREAFSLRHTTENKPHS
PKTPEPRRS
  

											 
																				

																		

										
											
Matching transcripts

												
																					PMS2-001 - ENSP00000265849 [100%] 
PMS2-006 - ENSP00000371758 [100%] 
PMS2-201 - ENSP00000392843 [100%] 

											 
																						PMS2-001 - ENSP00000265849 [97%] 
PMS2-201 - ENSP00000392843 [97%] 

											 
																						PMS2-001 - ENSP00000265849 [100%] 
PMS2-201 - ENSP00000392843 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
PMS2-001
PMS2-006
PMS2-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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