Expression of P4HA1 in cancer - Summary

GENERAL INFORMATION
Gene name	P4HA1
Gene description	Prolyl 4-hydroxylase, alpha polypeptide I
Protein class	Enzymes Plasma proteins Predicted secreted proteins
Predicted localization	Secreted
Number of transcripts	5
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HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):	Expressed in all
HPA (cell line):	Expressed in all
HPA (normal tissue):	Expressed in all

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Cytoplasmic expression in most tissues. Additional positivity in endothelial cells and extracellular matrix.

ANTIBODY IHC RELIABILITY
Data reliability description	Antibody staining mainly consistent with RNA expression data. Pending external verification.
Reliability score	Supported based on 2 antibodies. HPA007599 , HPA026593
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PROGNOSTIC SUMMARY
Prognostic marker in renal cancer (unfavourable), head and neck cancer (unfavourable), cervical cancer (unfavourable), pancreatic cancer (unfavourable), lung cancer (unfavourable) and breast cancer (unfavourable).
Renal cancer p<0.001 Head and neck cancer p<0.001 Cervical cancer p<0.001 Pancreatic cancer p<0.001 Lung cancer p<0.001 Breast cancer p<0.001 Show all

RNA EXPRESSION OVERVIEW
TCGA dataset RNA cancer category: Expressed in all

PROTEIN EXPRESSION

STAINING SUMMARY

HPA007599	HPA026593

GENE INFORMATION
Gene name	P4HA1 (HGNC Symbol)
Synonyms	C-P4Halpha(I), P4HA
Description	Prolyl 4-hydroxylase, alpha polypeptide I (HGNC Symbol)
Entrez gene summary	This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
Chromosome	10
Cytoband	q22.1
Chromosome location (bp)	73007217 - 73096974
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000122884 (version 83.38)
Entrez gene	5033
UniProt	P13674 (UniProt - Evidence at protein level)
neXtProt	NX_P13674
Antibodypedia	P4HA1 antibodies

PROTEIN BROWSER

P4HA1-001	P4HA1-002	P4HA1-201	P4HA1-202	P4HA1-203

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
P4HA1-001 ENSP00000307318 ENST00000307116	P13674 [Direct mapping] Prolyl 4-hydroxylase subunit alpha-1 Show all	Enzymes ENZYME proteins Oxidoreductases SPOCTOPUS predicted membrane proteins Predicted secreted proteins Secreted proteins predicted by MDSEC SignalP predicted secreted proteins Phobius predicted secreted proteins Plasma proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0004656 [procollagen-proline 4-dioxygenase activity] GO:0005506 [iron ion binding] GO:0005515 [protein binding] GO:0005739 [mitochondrion] GO:0005783 [endoplasmic reticulum] GO:0005788 [endoplasmic reticulum lumen] GO:0016020 [membrane] GO:0016491 [oxidoreductase activity] GO:0016702 [oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen] GO:0016705 [oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen] GO:0019511 [peptidyl-proline hydroxylation] GO:0031418 [L-ascorbic acid binding] GO:0043231 [intracellular membrane-bounded organelle] GO:0055114 [oxidation-reduction process] Show all	534 aa 61 kDa	Yes	0
P4HA1-002 ENSP00000362099 ENST00000373008	P13674 [Direct mapping] Prolyl 4-hydroxylase subunit alpha-1 Q5VSQ6 [Target identity:100%; Query identity:100%] Procollagen-proline, 2-oxoglutarate 4-dioxygenase (Proline 4-hydroxylase), alpha polypeptide I variant; Procollagen-proline, 2-oxoglutarate 4-dioxygenase (Proline 4-hydroxylase), alpha polypeptide I, isoform CRA_a; Procollagen-proline, 2-oxoglutarate 4-dioxygenase (Proline 4-hydroxylase), alpha polypeptide I, isoform CRA_b Show all	Enzymes ENZYME proteins Oxidoreductases SPOCTOPUS predicted membrane proteins Predicted secreted proteins Secreted proteins predicted by MDSEC SignalP predicted secreted proteins Phobius predicted secreted proteins Plasma proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0004656 [procollagen-proline 4-dioxygenase activity] GO:0005506 [iron ion binding] GO:0005515 [protein binding] GO:0005739 [mitochondrion] GO:0005783 [endoplasmic reticulum] GO:0005788 [endoplasmic reticulum lumen] GO:0016020 [membrane] GO:0016491 [oxidoreductase activity] GO:0016702 [oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen] GO:0016705 [oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen] GO:0019511 [peptidyl-proline hydroxylation] GO:0031418 [L-ascorbic acid binding] GO:0043231 [intracellular membrane-bounded organelle] GO:0055114 [oxidation-reduction process] Show all	534 aa 61 kDa	Yes	0
P4HA1-201 ENSP00000263556 ENST00000263556	P13674 [Direct mapping] Prolyl 4-hydroxylase subunit alpha-1 Q5VSQ6 [Target identity:100%; Query identity:100%] Procollagen-proline, 2-oxoglutarate 4-dioxygenase (Proline 4-hydroxylase), alpha polypeptide I variant; Procollagen-proline, 2-oxoglutarate 4-dioxygenase (Proline 4-hydroxylase), alpha polypeptide I, isoform CRA_a; Procollagen-proline, 2-oxoglutarate 4-dioxygenase (Proline 4-hydroxylase), alpha polypeptide I, isoform CRA_b Show all	Enzymes ENZYME proteins Oxidoreductases SPOCTOPUS predicted membrane proteins Predicted secreted proteins Secreted proteins predicted by MDSEC SignalP predicted secreted proteins Phobius predicted secreted proteins Plasma proteins Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0004656 [procollagen-proline 4-dioxygenase activity] GO:0005506 [iron ion binding] GO:0005515 [protein binding] GO:0005739 [mitochondrion] GO:0005783 [endoplasmic reticulum] GO:0005788 [endoplasmic reticulum lumen] GO:0016020 [membrane] GO:0016222 [procollagen-proline 4-dioxygenase complex] GO:0016491 [oxidoreductase activity] GO:0016702 [oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen] GO:0016705 [oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen] GO:0018401 [peptidyl-proline hydroxylation to 4-hydroxy-L-proline] GO:0019511 [peptidyl-proline hydroxylation] GO:0030199 [collagen fibril organization] GO:0031418 [L-ascorbic acid binding] GO:0043231 [intracellular membrane-bounded organelle] GO:0055114 [oxidation-reduction process] Show all	534 aa 61 kDa	Yes	0
P4HA1-202 ENSP00000378353 ENST00000394890	P13674 [Direct mapping] Prolyl 4-hydroxylase subunit alpha-1 Show all	Enzymes ENZYME proteins Oxidoreductases SPOCTOPUS predicted membrane proteins Predicted secreted proteins Secreted proteins predicted by MDSEC SignalP predicted secreted proteins Phobius predicted secreted proteins Plasma proteins Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0004656 [procollagen-proline 4-dioxygenase activity] GO:0005506 [iron ion binding] GO:0005515 [protein binding] GO:0005739 [mitochondrion] GO:0005783 [endoplasmic reticulum] GO:0005788 [endoplasmic reticulum lumen] GO:0016020 [membrane] GO:0016491 [oxidoreductase activity] GO:0016702 [oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen] GO:0016705 [oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen] GO:0019511 [peptidyl-proline hydroxylation] GO:0031418 [L-ascorbic acid binding] GO:0043231 [intracellular membrane-bounded organelle] GO:0055114 [oxidation-reduction process] Show all	534 aa 61 kDa	Yes	0
P4HA1-203 ENSP00000414464 ENST00000440381	P13674 [Direct mapping] Prolyl 4-hydroxylase subunit alpha-1 Show all	Enzymes ENZYME proteins Oxidoreductases SPOCTOPUS predicted membrane proteins Predicted secreted proteins Secreted proteins predicted by MDSEC SignalP predicted secreted proteins Phobius predicted secreted proteins Plasma proteins Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0004656 [procollagen-proline 4-dioxygenase activity] GO:0005506 [iron ion binding] GO:0005515 [protein binding] GO:0005739 [mitochondrion] GO:0005783 [endoplasmic reticulum] GO:0005788 [endoplasmic reticulum lumen] GO:0016020 [membrane] GO:0016491 [oxidoreductase activity] GO:0016702 [oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen] GO:0016705 [oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen] GO:0019511 [peptidyl-proline hydroxylation] GO:0031418 [L-ascorbic acid binding] GO:0043231 [intracellular membrane-bounded organelle] GO:0055114 [oxidation-reduction process] Show all	516 aa 59 kDa	Yes	0