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Antibody staining overlaps with antibody HPA025963.
Validated
Antibody staining overlaps with antibody HPA023252.
Standard validation
Approved
Approved
Figure description
Immunofluorescent staining of human cell line A-431 shows localization to nucleus & cytosol.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
Antibody dilution
1:50
1:7
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA023252
Antibody HPA023629
Antibody HPA025963
Antibody HPA031262
Antibody CAB068208
Antibody CAB068209
Antibody CAB068210
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA025963 across 74 cell types. Spearman correlation with HPA023629 across 74 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA023252 across 74 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA023252 across 74 cell types.
Standard validation
Supported
Supported
Supported
Supported
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human lateral ventricle shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic and nuclear positivity in neuronal cells.
Immunohistochemical staining of human hippocampus shows moderate cyoplasmic and nuclear positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human lateral ventricle shows strong cytoplasmic and nuclear positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic and nuclear positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows strong positivity in neuronal cells.
Expression
RNA: detected in 29 tissues Protein: detected in 11 cell types
RNA: detected in 29 tissues Protein: detected in 5 cell types
RNA: detected in 29 tissues Protein: detected in 19 cell types
RNA: detected in 29 tissues Protein: detected in 22 cell types
RNA: detected in 29 tissues Protein: detected in 10 cell types
RNA: detected in 29 tissues Protein: detected in 9 cell types
RNA: detected in 29 tissues Protein: detected in 16 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
HIER pH6
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:150
1:1200
1:200
1:800
1:7000
1:7000
1:7000
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Standard validation
Supported
Figure description
Subsets of neurons showed distinct positivity in cell bodies and dendrites. Main location of the positive neurons are layer 4 of the cerebral cortex.
WESTERN BLOT
Antibody HPA023252
Antibody HPA023629
Antibody HPA025963
Antibody HPA031262
Antibody CAB068208
Antibody CAB068209
Antibody CAB068210
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY411679)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY411679)
Target mass (kDa)
40.6
40.6
40.6, 11.8
40.6
40.6, 11.8
40.6, 11.8
40.6, 11.8
Antibody dilution
1:250
1:250
1:250
1:250
1:1000
1:1000
1:1000
PROTEIN ARRAY
Antibody HPA023252
Antibody HPA023629
Antibody HPA025963
Antibody HPA031262
Antibody CAB068208
Antibody CAB068209
Antibody CAB068210
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.