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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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PKN1
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PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

PKN1

Gene description

Protein kinase N1

Protein class

Enzymes
Predicted intracellular proteins

Predicted localization

Intracellular

Number of transcripts

7
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Expressed in all
HPA (cell line):Expressed in all
HPA (normal tissue):Expressed in all

Protein evidence

Evidence at protein level

Protein expression
normal tissue

General cytoplasmic expression.

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining mainly consistent with RNA expression data.

Reliability score

Approved based on 2 antibodies.
HPA003982 , CAB010278
SHOW MORE

PROGNOSTIC SUMMARY

Gene product is not prognostic.
Show all

RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Liver cancer

STAINING SUMMARY

HPA003982
CAB010278

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Most cancers showed moderate to strong cytoplasmic positivity.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Cancer cells generally displayed moderate immunoreactivity. Several breast cancers and gliomas were strongly stained.

GENE INFORMATION

Gene name

PKN1 (HGNC Symbol)

Synonyms

DBK, MGC46204, PAK1, PKN, PRK1, PRKCL1

Description

Protein kinase N1 (HGNC Symbol)

Entrez gene summary

The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Chromosome

19

Cytoband

p13.12

Chromosome location (bp)

14433053 - 14471867

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000123143 (version 83.38)

Entrez gene

5585

UniProt

Q16512 (UniProt - Evidence at protein level)

neXtProt

NX_Q16512

Antibodypedia

PKN1 antibodies

PROTEIN BROWSER

PKN1-001
PKN1-002
PKN1-004
PKN1-005
PKN1-006
PKN1-010
PKN1-013

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

PKN1-001
ENSP00000242783
ENST00000242783
Q16512 [Direct mapping]
Serine/threonine-protein kinase N1
Show all
Enzymes
   ENZYME proteins
   Transferases
   Kinases
   AGC Ser/Thr protein kinases
   MEMSAT-SVM predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
Predicted intracellular proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003682 [chromatin binding]
GO:0004672 [protein kinase activity]
GO:0004674 [protein serine/threonine kinase activity]
GO:0004697 [protein kinase C activity]
GO:0005080 [protein kinase C binding]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005737 [cytoplasm]
GO:0005768 [endosome]
GO:0005829 [cytosol]
GO:0005886 [plasma membrane]
GO:0006351 [transcription, DNA-templated]
GO:0006357 [regulation of transcription from RNA polymerase II promoter]
GO:0006468 [protein phosphorylation]
GO:0007165 [signal transduction]
GO:0007257 [activation of JUN kinase activity]
GO:0007264 [small GTPase mediated signal transduction]
GO:0010631 [epithelial cell migration]
GO:0017049 [GTP-Rho binding]
GO:0030374 [ligand-dependent nuclear receptor transcription coactivator activity]
GO:0030496 [midbody]
GO:0032154 [cleavage furrow]
GO:0035402 [histone kinase activity (H3-T11 specific)]
GO:0035407 [histone H3-T11 phosphorylation]
GO:0042393 [histone binding]
GO:0042826 [histone deacetylase binding]
GO:0048365 [Rac GTPase binding]
GO:0050681 [androgen receptor binding]
GO:2000145 [regulation of cell motility]
Show all
942 aa
103.9 kDa
No 0
PKN1-002
ENSP00000343325
ENST00000342216
Q16512 [Direct mapping]
Serine/threonine-protein kinase N1
Show all
Enzymes
   ENZYME proteins
   Transferases
   Kinases
   AGC Ser/Thr protein kinases
   MEMSAT-SVM predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
Predicted intracellular proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001782 [B cell homeostasis]
GO:0001783 [B cell apoptotic process]
GO:0001933 [negative regulation of protein phosphorylation]
GO:0002634 [regulation of germinal center formation]
GO:0002637 [regulation of immunoglobulin production]
GO:0003014 [renal system process]
GO:0003682 [chromatin binding]
GO:0004672 [protein kinase activity]
GO:0004674 [protein serine/threonine kinase activity]
GO:0004697 [protein kinase C activity]
GO:0005080 [protein kinase C binding]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005737 [cytoplasm]
GO:0005768 [endosome]
GO:0005829 [cytosol]
GO:0005886 [plasma membrane]
GO:0006351 [transcription, DNA-templated]
GO:0006357 [regulation of transcription from RNA polymerase II promoter]
GO:0006468 [protein phosphorylation]
GO:0006469 [negative regulation of protein kinase activity]
GO:0006972 [hyperosmotic response]
GO:0007165 [signal transduction]
GO:0007257 [activation of JUN kinase activity]
GO:0007264 [small GTPase mediated signal transduction]
GO:0010631 [epithelial cell migration]
GO:0016023 [cytoplasmic membrane-bounded vesicle]
GO:0017049 [GTP-Rho binding]
GO:0030374 [ligand-dependent nuclear receptor transcription coactivator activity]
GO:0030496 [midbody]
GO:0030889 [negative regulation of B cell proliferation]
GO:0032154 [cleavage furrow]
GO:0035402 [histone kinase activity (H3-T11 specific)]
GO:0035407 [histone H3-T11 phosphorylation]
GO:0042393 [histone binding]
GO:0042826 [histone deacetylase binding]
GO:0048365 [Rac GTPase binding]
GO:0048536 [spleen development]
GO:0050681 [androgen receptor binding]
GO:2000145 [regulation of cell motility]
Show all
948 aa
104.7 kDa
No 0
PKN1-004
ENSP00000467422
ENST00000585619
K7EPK3 [Direct mapping]
Serine/threonine-protein kinase N1
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
15 aa
1.7 kDa
No 0
PKN1-005
ENSP00000465842
ENST00000586557
K7EKY9 [Direct mapping]
Serine/threonine-protein kinase N1
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0007165 [signal transduction]
Show all
256 aa
28.3 kDa
No 0
PKN1-006
ENSP00000466367
ENST00000590097
K7EM57 [Direct mapping]
Serine/threonine-protein kinase N1
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0007165 [signal transduction]
Show all
263 aa
29 kDa
No 0
PKN1-010
ENSP00000466819
ENST00000586237
K7EN76 [Direct mapping]
Serine/threonine-protein kinase N1
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
151 aa
15.7 kDa
No 0
PKN1-013
ENSP00000468497
ENST00000592960
K7ES10 [Direct mapping]
Serine/threonine-protein kinase N1
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
226 aa
24.2 kDa
No 0

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