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CDKN1A

ANTIBODY INFORMATION
 
 

Antibody HPA005946

  

Antibody CAB000064

  

Antibody CAB069401

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Oncogene Research Products
   AbFrontier
  

Product name

 HPA005946   OP64   LF-MA0289  

Host species

 Rabbit   Mouse   Mouse  

Clonality

 pAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known   Protein A/G  

Other gene match

      

Released in version

 16   1   14  

References

      

Proper citation

 Atlas Antibodies Cat#HPA005946, RRID:AB_2667477     ABFrontier Cat#LF-MA0289, RRID:AB_1620493  

VALIDATION SUMMARY
 
 

Antibody HPA005946

  

Antibody CAB000064

  

Antibody CAB069401

  
Immunocytochemistry

Supported      
Immunohistochemistry

  Supported   Supported  
Western blot

Uncertain   Supported   Supported  
Protein array

Supported      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA005946

  

Antibody CAB000064

  

Antibody CAB069401

  

Standard validation

 Supported      

     

Figure description

 Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.      

Antibody dilution

 1:10      

Literature conformity

 The subcellular location is supported by literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA005946

  

Antibody CAB000064

  

Antibody CAB069401

  

Standard validation

   Supported   Supported  

     

Figure description

   Immunohistochemical staining of human cervix, uterine shows nuclear positivity in squamous cells.   Immunohistochemical staining of human urinary bladder shows strong nuclear positivity in urothelial cells.  

Expression

   RNA: detected in 37 tissues
Protein: detected in 34 cell types		   RNA: detected in 37 tissues
Protein: detected in 56 cell types		  

Retrieval

   HIER pH6   HIER pH6  

Antibody dilution

   1:150   1:1000  

Literature conformity

   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA005946

  

Antibody CAB000064

  

Antibody CAB069401

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 18.1   18.1   18.1  

Antibody dilution

 1:250   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA005946

  

Antibody CAB000064

  

Antibody CAB069401

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:2000      

ANTIGEN INFORMATION
 
 

Antibody HPA005946

  

Antibody CAB000064

  

Antibody CAB069401

  

Antigen

 Recombinant protein fragment   Not known   Recombinant protein  

Length (aa)

 111      

Antigen sequence

 
ARERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRR
PGTSPALLQGTAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQ
TSMTDFYHSKR
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					CDKN1A-001 - ENSP00000244741 [100%] 
CDKN1A-002 - ENSP00000362815 [100%] 
CDKN1A-201 - ENSP00000384849 [100%] 
CDKN1A-202 - ENSP00000409259 [100%] 
CDKN1A-203 - ENSP00000482768 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CDKN1A-001
CDKN1A-002
CDKN1A-201
CDKN1A-202
CDKN1A-203
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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