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Antibody staining overlaps with antibody HPA027898. Antibody staining overlaps with antibody HPA027897.
Validated
Antibody staining overlaps with antibody HPA027898. Antibody staining overlaps with antibody HPA027896.
Validated
Antibody staining overlaps with antibody HPA027897. Antibody staining overlaps with antibody HPA027896.
Standard validation
Supported
Supported
Supported
Figure description
Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear membrane.
Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear membrane.
Immunofluorescent staining of human cell line A-431 shows localization to nuclear membrane.
Antibody dilution
1:20
1:50
1:50
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA027896
Antibody HPA027897
Antibody HPA027898
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human cerebellum shows strong nuclear and nuclear membranous positivity.
Immunohistochemical staining of human cerebral cortex shows strong nuclear membranous positivity in neuronal cells.
Immunohistochemical staining of human breast shows strong nuclear membrane and cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 68 cell types
RNA: detected in 37 tissues Protein: detected in 76 cell types
RNA: detected in 37 tissues Protein: detected in 71 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:400
1:50
1:150
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA027896
Antibody HPA027897
Antibody HPA027898
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
153.9, 149.4
157.3, 153.9, 149.4
157.3, 153.9, 149.4
Antibody dilution
1:250
1:250
1:250
PROTEIN ARRAY
Antibody HPA027896
Antibody HPA027897
Antibody HPA027898
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
