Fields »
ABOUT
HELP
BLOG
GPR108

ANTIBODY INFORMATION
 
 

Antibody HPA041924

  

Antibody HPA041951

  

Antibody HPA063863

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA041924   HPA041951   HPA063863  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 8   8   13  

References

      

Proper citation

 Atlas Antibodies Cat#HPA041924, RRID:AB_10804122   Atlas Antibodies Cat#HPA041951, RRID:AB_10795385   Atlas Antibodies Cat#HPA063863, RRID:AB_2685135  

VALIDATION SUMMARY
 
 

Antibody HPA041924

  

Antibody HPA041951

  

Antibody HPA063863

  
Immunocytochemistry

Approved     Approved  
Immunohistochemistry

Uncertain   Uncertain    
Western blot

Uncertain   Uncertain   Supported  
Protein array

Approved   Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA041924

  

Antibody HPA041951

  

Antibody HPA063863

  

Standard validation

 Approved     Approved  

     

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus & vesicles.     Immunofluorescent staining of human cell line SiHa shows localization to the Golgi apparatus.  

Antibody dilution

 1:156     1:38  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.     The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA041924

  

Antibody HPA041951

  

Antibody HPA063863

  

Standard validation

 Uncertain   Uncertain    

     

Figure description

 Immunohistochemical staining of human placenta shows cytoplasmic positivity in trophoblastic cells.   Immunohistochemical staining of human placenta shows cytopalsmic positivity in trophoblastic cells.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 41 cell types		   RNA: detected in 37 tissues
Protein: detected in 29 cell types		    

Retrieval

 HIER pH6   HIER pH6    

Antibody dilution

 1:100   1:15    

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.    

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA041924

  

Antibody HPA041951

  

Antibody HPA063863

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 60.6, 24.3   60.6, 24.3   60.6, 34.3, 20.2  

Antibody dilution

 1:250   1:250   1:88  

PROTEIN ARRAY
 
 

Antibody HPA041924

  

Antibody HPA041951

  

Antibody HPA063863

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:6000   1:12000   1:1550  

ANTIGEN INFORMATION
 
 

Antibody HPA041924

  

Antibody HPA041951

  

Antibody HPA063863

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 86   79   47  

Antigen sequence

 
VGFSLSRVRSGRVRSYSTRDFQDCPLQKNSSSFLVLFLINTKDLQVQVRK
YGEQKTLFIFPGLLPEAPSKPGLPKPQATVPRKVDG
  
SKPKSTPAVIQGPSGKDEDLVLGLSHLNNSYNFSFHVVIGSQAEEGQYSL
NFHNCNNSVPGKEHPFDITVMIREKNPDG
  
QPTGNNPYLQLPQEDEEDVQMEQVMTDSGFREGLSKVNKTASGRELL
 

Matching transcripts

 GPR108-001 - ENSP00000264080 [100%]
GPR108-005 - ENSP00000469797 [83%]
  GPR108-001 - ENSP00000264080 [99%]
GPR108-005 - ENSP00000469797 [86%]
  GPR108-001 - ENSP00000264080 [100%]
GPR108-002 - ENSP00000401284 [100%]
GPR108-011 - ENSP00000470823 [100%]
 

Other gene match

      
ANTIGEN VIEW
GPR108-001
GPR108-002
GPR108-005
GPR108-011
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.