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Immunohistochemical staining of human testis shows strong nuclear positivity in subset of cells in seminiferus ducts.
Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.
Expression
RNA: detected in 37 tissues Protein: detected in 45 cell types
RNA: detected in 37 tissues Protein: detected in 23 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:40
1:10
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA036084
Antibody HPA064381
Antibody CAB003808
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
44.9
44.9
44.9, 11.2
Antibody dilution
1:250
1:210
1:500
PROTEIN ARRAY
Antibody HPA036084
Antibody HPA064381
Antibody CAB003808
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
