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SLC35E1

ANTIBODY INFORMATION
 
 

Antibody HPA016570

  

Antibody HPA062214

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA016570   HPA062214  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 4   14  

References

    

Proper citation

 Atlas Antibodies Cat#HPA016570, RRID:AB_2190214   Atlas Antibodies Cat#HPA062214, RRID:AB_2684713  

VALIDATION SUMMARY
 
 

Antibody HPA016570

  

Antibody HPA062214

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry

Approved    
Western blot

Supported   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA016570

  

Antibody HPA062214

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA062214.		  
Validated

Antibody staining overlaps with antibody HPA016570.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.   Immunofluorescent staining of human cell line MCF7 shows localization to the Golgi apparatus.  

Antibody dilution

 1:62   1:74  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA016570

  

Antibody HPA062214

  

Standard validation

 Approved    

   

Figure description

 Immunohistochemical staining of human kidney shows cytoplasmic positivity in cells in tubules.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 26 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:300    

Literature conformity

 No avaliable gene/protein characterization data.    

RNA consistency

 Mainly not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA016570

  

Antibody HPA062214

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 44.8, 38.3   44.8, 38.3  

Antibody dilution

 1:250   1:170  

PROTEIN ARRAY
 
 

Antibody HPA016570

  

Antibody HPA062214

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:2950  

ANTIGEN INFORMATION
 
 

Antibody HPA016570

  

Antibody HPA062214

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 78   24  

Antigen sequence

 
NKTKYDANQQARKHLLPVTTADLSSKERHRSPLEKPHNGLLFPQHGDYQY
GRNNILTDHFQYSRQSYPNSYSLNRYDV
  
GPSPHPSSGPLLPPRFYPRYVLPL
 

Matching transcripts

 SLC35E1-001 - ENSP00000470652 [100%]
SLC35E1-004 - ENSP00000400435 [100%]
  SLC35E1-001 - ENSP00000470652 [100%]
SLC35E1-004 - ENSP00000400435 [100%]
 

Other gene match

    
ANTIGEN VIEW
SLC35E1-001
SLC35E1-004
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.