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HIP1

ANTIBODY INFORMATION
 
 

Antibody HPA013606

  

Antibody HPA017964

  

Antibody CAB015334

  

Antibody CAB016402

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
   Santa Cruz Biotechnology
  

Product name

 HPA013606   HPA017964   sc-33601   sc-47754  

Host species

 Rabbit   Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G   Protein A/G  

Other gene match

        

Released in version

 4   4   4   4  

References

 1        

Proper citation

 Atlas Antibodies Cat#HPA013606, RRID:AB_1850717   Atlas Antibodies Cat#HPA017964, RRID:AB_1850719   Santa Cruz Biotechnology Cat#sc-33601, RRID:AB_2117565   Santa Cruz Biotechnology Cat#sc-47754, RRID:AB_627725  

VALIDATION SUMMARY
 
 

Antibody HPA013606

  

Antibody HPA017964

  

Antibody CAB015334

  

Antibody CAB016402

  
Immunocytochemistry

Supported     Supported    
Immunohistochemistry


Validated
  

Validated

Validated
  

Validated
  

Validated
  
Western blot

Uncertain   Supported   Supported   Supported  
Protein array

Supported   Supported      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA013606

  

Antibody HPA017964

  

Antibody CAB015334

  

Antibody CAB016402

  

Standard validation

 Supported     Supported    

       

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.     Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.    

Antibody dilution

 1:222     1:23    

Literature conformity

 The subcellular location is supported by literature.     The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA013606

  

Antibody HPA017964

  

Antibody CAB015334

  

Antibody CAB016402

  

Formal validation: Orthogonal

  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 
 
 

Figure description

   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 42 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 42 cell lines.  
 

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		      

 
     

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA017964 across 43 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA013606 across 43 cell lines.		      
 

Standard validation

 Supported   Supported   Supported   Supported  

       

Figure description

 Immunohistochemical staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.   Immunohistochemical staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.   Immunohistochemical staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.   Immunohistochemical staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.  

Expression

 RNA: detected in 35 tissues
Protein: detected in 67 cell types		   RNA: detected in 35 tissues
Protein: detected in 69 cell types		   RNA: detected in 35 tissues
Protein: detected in 72 cell types		   RNA: detected in 35 tissues
Protein: detected in 71 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:250   1:600   1:90   1:50  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA013606

  

Antibody HPA017964

  

Antibody CAB015334

  

Antibody CAB016402

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

       

Figure description

   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 116.2, 113.1, 110.7   116.2, 113.1, 110.7   116.2, 113.1, 110.7, 16.9   116.2, 113.1, 110.7, 16.9  

Antibody dilution

 1:250   1:250   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA013606

  

Antibody HPA017964

  

Antibody CAB015334

  

Antibody CAB016402

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:3000   1:3000      

ANTIGEN INFORMATION
 
 

Antibody HPA013606

  

Antibody HPA017964

  

Antibody CAB015334

  

Antibody CAB016402

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein   Not known  

Length (aa)

 150   140      

Antigen sequence

 
NFLRASALSEHISPVVVIPAEASSPDSEPVLEKDDLMDMDASQQNLFDNK
FDDIFGSSFSSDPFNFNSQNGVNKDEKDHLIERLYREISGLKAQLENMKT
ESQRVVLQLKGHVSELEADLAEQQHLRQQAADDCEFLRAELDELRRQRED
  
QVIQDALNQLEEPPLISCAGSADHLLSTVTSISSCIEQLEKSWSQYLACP
EDISGLLHSITLLAHLTSDAIAHGATTCLRAPPEPADSLTEACKQYGRET
LAYLASLEEEGSLENADSTAMRNCLSKIKAIGEELLPRGL
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					HIP1-001 - ENSP00000336747 [100%] 
HIP1-002 - ENSP00000410300 [100%] 
HIP1-201 - ENSP00000484528 [100%] 

											 
																						HIP1-001 - ENSP00000336747 [100%] 
HIP1-002 - ENSP00000410300 [100%] 
HIP1-201 - ENSP00000484528 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
HIP1-001
HIP1-002
HIP1-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA013606

												
										 
																				
											
											
											
Antibody HPA017964

												
										 
																				
											
											
											
Antibody CAB015334

												
										 
																				
											
											
											
Antibody CAB016402

												
										 
																		

																	

										
											
1.

												
																						Clathrin light chains are required for the gyrating-clathrin recycling pathway and thereby promote cell migration

	Majeed SR et al

	Nat Commun 2014;5:3891

			
											 
																						
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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