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PSMA1

ANTIBODY INFORMATION
 
 

Antibody HPA037646

  

Antibody HPA043891

  

Antibody CAB033765

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA037646   HPA043891   sc-67046  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 7   12   13  

References

      

Proper citation

 Atlas Antibodies Cat#HPA037646, RRID:AB_10670386   Atlas Antibodies Cat#HPA043891, RRID:AB_2678718   Santa Cruz Biotechnology Cat#sc-67046, RRID:AB_2253026  

VALIDATION SUMMARY
 
 

Antibody HPA037646

  

Antibody HPA043891

  

Antibody CAB033765

  
Immunocytochemistry

Supported   Supported    
Immunohistochemistry


Validated
  

Validated
   Supported  
Western blot

Supported   Supported   Supported  
Protein array

Approved   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA037646

  

Antibody HPA043891

  

Antibody CAB033765

  

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.   Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.    

Antibody dilution

 1:105   1:9    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA037646

  

Antibody HPA043891

  

Antibody CAB033765

  

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		    

 
   

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA043891 across 45 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA037646 across 45 cell lines.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human testis shows distinct cytoplasmic and nuclear positivity in cells in seminiferus ducts and Leydig cells.   Immunohistochemical staining of human testis shows strong nuclear and cytoplasmic positivity in cells in seminiferus ducts and Leydig cells.   Immunohistochemical staining of human testis shows distinct cytoplasmic and nuclear positivity in cells in seminiferus ducts and Leydig cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 80 cell types		   RNA: detected in 37 tissues
Protein: detected in 69 cell types		   RNA: detected in 37 tissues
Protein: detected in 77 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:2250   1:20   1:100  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA037646

  

Antibody HPA043891

  

Antibody CAB033765

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 30.2, 29.6, 26.5   30.2, 29.6, 26.5   30.2, 29.6, 26.5, 1.4, 0.3  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA037646

  

Antibody HPA043891

  

Antibody CAB033765

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA037646

  

Antibody HPA043891

  

Antibody CAB033765

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 89   77    

Antigen sequence

 
SQSARTYLERHMSEFMECNLNELVKHGLRALRETLPAEQDLTTKNVSIGI
VGKDLEFTIYDDDDVSPFLEGLEERPQRKAQPAQPADEP
  
RQECLDSRFVFDRPLPVSRLVSLIGSKTQIPTQRYGRRPYGVGLLIAGYD
DMGPHIFQTCPSANYFDCRAMSIGARS
  

											 
																				

																		

										
											
Matching transcripts

												
																					PSMA1-001 - ENSP00000379676 [100%] 
PSMA1-002 - ENSP00000414359 [100%] 
PSMA1-004 - ENSP00000441166 [100%] 

											 
																						PSMA1-001 - ENSP00000379676 [100%] 
PSMA1-002 - ENSP00000414359 [100%] 
PSMA1-004 - ENSP00000441166 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
PSMA1-001
PSMA1-002
PSMA1-004
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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