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SUMF2

ANTIBODY INFORMATION
 
 

Antibody HPA024040

  

Antibody HPA074892

  

Antibody CAB025743

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   R&D Systems
  

Product name

 HPA024040   HPA074892   MAB3594  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 5   16   6  

References

      

Proper citation

 Atlas Antibodies Cat#HPA024040, RRID:AB_1857651      

VALIDATION SUMMARY
 
 

Antibody HPA024040

  

Antibody HPA074892

  

Antibody CAB025743

  
Immunocytochemistry

  Uncertain    
Immunohistochemistry


Validated
     Supported  
Western blot

Uncertain     Supported  
Protein array

Approved   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA024040

  

Antibody HPA074892

  

Antibody CAB025743

  

Standard validation

   Uncertain    

     

Figure description

   Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & vesicles.    

Antibody dilution

   1:179    

Literature conformity

   The subcellular location is not consistent with literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA024040

  

Antibody HPA074892

  

Antibody CAB025743

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		      

     

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.      
 

Standard validation

 Supported     Supported  

     

Figure description

 Immunohistochemical staining of human gallbladder shows strong cytoplasmic positivity in glandular cells.     Immunohistochemical staining of human gallbladder shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 38 cell types		     RNA: detected in 37 tissues
Protein: detected in 67 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:10     1:20  

Literature conformity

 Consistent with gene/protein characterization data.     Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.     Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA024040

  

Antibody HPA074892

  

Antibody CAB025743

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		     Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

     Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 39.7, 39.5, 35.9, 34.1, 34, 33.4, 26, 25.8     39.7, 39.5, 35.9, 34.1, 34, 33.4, 26, 25.8, 24.1  

Antibody dilution

 1:250     1:500  

PROTEIN ARRAY
 
 

Antibody HPA024040

  

Antibody HPA074892

  

Antibody CAB025743

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500   1:7150    

ANTIGEN INFORMATION
 
 

Antibody HPA024040

  

Antibody HPA074892

  

Antibody CAB025743

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 94   92    

Antigen sequence

 
ATSMVQLQGGRFLMGTNSPDSRDGEGPVREATVKPFAIDIFPVTNKDFRD
FVREKKYRTEAEMFGWSFVFEDFVSDELRNKATQPMKSVLWWLP
  
PKGDKAEDGFHGVSPVNAFPAQNNYGLYDLLGNVWEWTASPYQAAEQDMR
VLRGASWIDTADGSANHRARVTTRMGNTPDSASDNLGFRCAA
  

											 
																				

																		

										
											
Matching transcripts

												
																					SUMF2-001 - ENSP00000400922 [99%] 
SUMF2-009 - ENSP00000415989 [99%] 
SUMF2-013 - ENSP00000406445 [99%] 
SUMF2-015 - ENSP00000341938 [99%] 
SUMF2-003 - ENSP00000414434 [96%] 
SUMF2-002 - ENSP00000378824 [91%] 
SUMF2-010 - ENSP00000378823 [91%] 
SUMF2-012 - ENSP00000410796 [82%] 

											 
																						SUMF2-001 - ENSP00000400922 [100%] 
SUMF2-002 - ENSP00000378824 [100%] 
SUMF2-009 - ENSP00000415989 [100%] 
SUMF2-010 - ENSP00000378823 [100%] 
SUMF2-201 - ENSP00000275607 [100%] 
SUMF2-012 - ENSP00000410796 [95%] 
SUMF2-013 - ENSP00000406445 [80%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
SUMF2-001
SUMF2-002
SUMF2-003
SUMF2-009
SUMF2-010
SUMF2-012
SUMF2-013
SUMF2-015
SUMF2-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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