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CDH15

ANTIBODY INFORMATION
 
 

Antibody HPA009139

  

Antibody HPA070961

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA009139   HPA070961  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 3   14  

References

    

Proper citation

 Atlas Antibodies Cat#HPA009139, RRID:AB_1078372   Atlas Antibodies Cat#HPA070961, RRID:AB_2686328  

VALIDATION SUMMARY
 
 

Antibody HPA009139

  

Antibody HPA070961

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry


Validated
    
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA009139

  

Antibody HPA070961

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA070961.		  
Validated

Antibody staining overlaps with antibody HPA009139.		  
 

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line RH-30 shows localization to cytosol & the Golgi apparatus.   Immunofluorescent staining of human cell line RH-30 shows localization to cytosol & the Golgi apparatus.  

Antibody dilution

 1:44   1:108  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA009139

  

Antibody HPA070961

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    

   

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.    
 

Standard validation

 Supported    

   

Figure description

 Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.    

Expression

 RNA: detected in 2 tissues
Protein: detected in 9 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:50    

Literature conformity

 Consistent with extensive gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA009139

  

Antibody HPA070961

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 88.9   88.9  

Antibody dilution

 1:250   1:360  

PROTEIN ARRAY
 
 

Antibody HPA009139

  

Antibody HPA070961

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:4300  

ANTIGEN INFORMATION
 
 

Antibody HPA009139

  

Antibody HPA070961

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 146   75  

Antigen sequence

 
TFSARDPDTEQLQRLSYSKDYDPEDWLQVDAATGRIQTQHVLSPASPFLK
GGWYRAIVLAQDDASQPRTATGTLSIEILEVNDHAPVLAPPPPGSLCSEP
HQGPGLLLGATDEDLPPHGAPFHFQLSPRLPELGRNWSLSQVNVSH
  
LSIVKALDYESCEHYELKVSVQNEAPLQAAALRAERGQAKVRVHVQDTNE
PPVFQENPLRTSLAEGAPPGTLVAT
 

Matching transcripts

 CDH15-001 - ENSP00000289746 [100%]
  CDH15-001 - ENSP00000289746 [100%]
 

Other gene match

    
ANTIGEN VIEW
CDH15-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.