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Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 24 cell types
RNA: detected in 37 tissues Protein: detected in 39 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:25
1:7
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA009647
Antibody HPA013159
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400183)
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
104.8, 104.6, 95.4, 95.1, 90.9, 76.9
104.8, 104.6, 95.4, 95.1, 90.9, 76.9, 75.9
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA009647
Antibody HPA013159
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
