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CAMSAP1

ANTIBODY INFORMATION
 
 

Antibody HPA020527

  

Antibody HPA024161

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA020527   HPA024161  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 16   5  

References

    

Proper citation

   Atlas Antibodies Cat#HPA024161, RRID:AB_1845967  

VALIDATION SUMMARY
 
 

Antibody HPA020527

  

Antibody HPA024161

  
Immunocytochemistry

Uncertain    
Immunohistochemistry

  Approved  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA020527

  

Antibody HPA024161

  

Standard validation

 Uncertain    

   

Figure description

 Immunofluorescent staining of human cell line SH-SY5Y shows localization to cytosol & mitotic spindle.    

Antibody dilution

 1:116    

Literature conformity

 The subcellular location is not consistent with literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA020527

  

Antibody HPA024161

  

Standard validation

   Approved  

   

Figure description

   Immunohistochemical staining of human cerebral cortex shows strong positivity in neuropil.  

Expression

   RNA: detected in 37 tissues
Protein: detected in 58 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:500  

Literature conformity

   Partly consistent with gene/protein characterization data.  

RNA consistency

   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA020527

  

Antibody HPA024161

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 179.3, 178, 146.5   179.3, 178, 146.5  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA020527

  

Antibody HPA024161

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA020527

  

Antibody HPA024161

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 114   99  

Antigen sequence

 
AGEVCGGPLALGGFDPFPQGPSTDGFFLHVGRADEDTEGRLYVSCSKSPN
SHDSEPWTLLRQDSDSDVVDIEEAEHDFMGEAHPVVFSRYIGEEESAKLQ
EDMKVKEHEDKDDA
  
TPTDPGLDSALEPSGDPHGKCLFDSYRLHDESNQRTLTLSSSKDANILSE
QMSLKEVLDASVKEVGSSSSDVSGKESVPVEEPLRSRASLIEVDLSDLK
 

Matching transcripts

 CAMSAP1-001 - ENSP00000374183 [100%]
CAMSAP1-002 - ENSP00000386420 [100%]
CAMSAP1-010 - ENSP00000312463 [100%]
  CAMSAP1-001 - ENSP00000374183 [100%]
CAMSAP1-002 - ENSP00000386420 [100%]
CAMSAP1-010 - ENSP00000312463 [100%]
 

Other gene match

    
ANTIGEN VIEW
CAMSAP1-001
CAMSAP1-002
CAMSAP1-010
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.