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Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA020934 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA020896 across 46 cell lines.
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA020934 across 73 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA020896 across 73 cell types.
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in tubules.
Expression
RNA: detected in 36 tissues Protein: detected in 60 cell types
RNA: detected in 36 tissues Protein: detected in 35 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:25
1:600
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA020896
Antibody HPA020934
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403289)
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
46.5, 22.4, 20.3
46.5
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA020896
Antibody HPA020934
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
