NPAS1 - Antibodies - The Human Protein Atlas

We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.

NPAS1

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA043364

Antibody HPA072259

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA043364

HPA072259

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA072259, RRID:AB_2686500

VALIDATION SUMMARY

Antibody HPA043364

Antibody HPA072259

Immunocytochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA043364

Antibody HPA072259

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.

Antibody dilution

1:43

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA043364

Antibody HPA072259

Standard validation

Approved

Figure description

Mostly dendritic staining in a few areas throughout the brain.

WESTERN BLOT

Antibody HPA043364

Antibody HPA072259

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.

Figure description

Target mass (kDa)

62.7, 44.1, 25, 22.9

62.7, 44.1

Antibody dilution

1:250

1:140

PROTEIN ARRAY

Antibody HPA043364

Antibody HPA072259

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:6000

ANTIGEN INFORMATION

Antibody HPA043364

Antibody HPA072259

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

ALGHTLPPAPLAELPLHGHMIVFRLSLGLTILACESRVSDHMDLGPSELV
GRSCYQFVHGQDATRIRQSHVDLLDKGQVMTGYYR

VLWVSHVLSQAEGGQTPLDAFQLPASVACEEASSPGPEPTEPEPPTEGKQ
AAPAENEAPQTQGQRIKV

Matching transcripts

NPAS1-001 - ENSP00000469142 [100%]
NPAS1-002 - ENSP00000472679 [100%]
NPAS1-003 - ENSP00000405290 [100%]
NPAS1-004 - ENSP00000398689 [85%]
NPAS1-006 - ENSP00000472075 [85%]

NPAS1-001 - ENSP00000469142 [99%]
NPAS1-002 - ENSP00000472679 [99%]
NPAS1-003 - ENSP00000405290 [99%]

Other gene match

ANTIGEN VIEW

NPAS1-001	NPAS1-002	NPAS1-003	NPAS1-004	NPAS1-006

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.