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DKC1

ANTIBODY INFORMATION
 
 

Antibody HPA000166

  

Antibody HPA000447

  

Antibody HPA001022

  

Antibody CAB033086

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA000166   HPA000447   HPA001022   sc-48794  

Host species

 Rabbit   Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

        

Released in version

 1   1   12   13  

References

 4   1      

Proper citation

 Atlas Antibodies Cat#HPA000166, RRID:AB_1078669   Atlas Antibodies Cat#HPA000447, RRID:AB_1078667   Atlas Antibodies Cat#HPA001022, RRID:AB_2666175   Santa Cruz Biotechnology Cat#sc-48794, RRID:AB_2091314  

VALIDATION SUMMARY
 
 

Antibody HPA000166

  

Antibody HPA000447

  

Antibody HPA001022

  

Antibody CAB033086

  
Immunocytochemistry

    Supported    
Immunohistochemistry

Supported   Supported     Supported  
Western blot

Supported   Supported   Supported   Supported  
Protein array

Supported   Supported   Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA000166

  

Antibody HPA000447

  

Antibody HPA001022

  

Antibody CAB033086

  

Standard validation

     Supported    

       

Figure description

     Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & nucleoli fibrillar center.    

Antibody dilution

     1:50    

Literature conformity

     The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA000166

  

Antibody HPA000447

  

Antibody HPA001022

  

Antibody CAB033086

  

Standard validation

 Supported   Supported     Supported  

       

Figure description

 Immunohistochemical staining of human cerebral cortex shows strong nucleolar positivity in neuronal cells.   Immunohistochemical staining of human gall bladder shows distinct nuclear positivity in glandular cells.     Immunohistochemical staining of human rectum shows moderate nuclear positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 48 cell types		   RNA: detected in 37 tissues
Protein: detected in 76 cell types		     RNA: detected in 37 tissues
Protein: detected in 70 cell types		  

Retrieval

 HIER pH9   HIER pH6     HIER pH6  

Antibody dilution

 1:200   1:250     1:300  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.     Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.     Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA000166

  

Antibody HPA000447

  

Antibody HPA001022

  

Antibody CAB033086

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

       

Figure description

 Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 57.7, 47.6   57.7, 47.6, 29.8, 25.7   57.7, 47.6, 29.8, 25.7   57.7, 47.6, 29.8, 25.7  

Antibody dilution

 1:250   1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA000166

  

Antibody HPA000447

  

Antibody HPA001022

  

Antibody CAB033086

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:1000   1:2000    

ANTIGEN INFORMATION
 
 

Antibody HPA000166

  

Antibody HPA000447

  

Antibody HPA001022

  

Antibody CAB033086

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 117   108   108    

Antigen sequence

 
EAGTYIRTLCVHLGLLLGVGGQMQELRRVRSGVMSEKDHMVTMHDVLDAQ
WLYDNHKDESYLRRVVYPLEKLLTSHKRLVMKDSAVNAICYGAKIMLPGV
LRYEDGIEVNQEIVVIT
  
KERKSLPEEDVAEIQHAEEFLIKPESKVAKLDTSQWPLLLKNFDKLNVRT
THYTPLACGSNPLKREIGDYIRTGFINLDKPSNPSSHEVVAWIRRILRVE
KTGHSGTL
  
KERKSLPEEDVAEIQHAEEFLIKPESKVAKLDTSQWPLLLKNFDKLNVRT
THYTPLACGSNPLKREIGDYIRTGFINLDKPSNPSSHEVVAWIRRILRVE
KTGHSGTL
  

											 
																				

																		

										
											
Matching transcripts

												
																					DKC1-001 - ENSP00000358563 [100%] 
DKC1-201 - ENSP00000478387 [100%] 

											 
																						DKC1-001 - ENSP00000358563 [100%] 
DKC1-008 - ENSP00000400542 [100%] 
DKC1-010 - ENSP00000395693 [100%] 
DKC1-201 - ENSP00000478387 [100%] 

											 
																						DKC1-001 - ENSP00000358563 [100%] 
DKC1-008 - ENSP00000400542 [100%] 
DKC1-010 - ENSP00000395693 [100%] 
DKC1-201 - ENSP00000478387 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
DKC1-001
DKC1-008
DKC1-010
DKC1-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA000166

												
										 
																				
											
											
											
Antibody HPA000447

												
										 
																				
											
											
											
Antibody HPA001022

												
										 
																				
											
											
											
Antibody CAB033086

												
										 
																		

																	

										
											
1.

												
																						Relationship between dyskerin expression and telomerase activity in human breast cancer

	Montanaro L et al

	Cell Oncol 2008;30(6):483-90

			
											 
																							snoRNPs Regulate Telomerase Activity in Neuroblastoma and Are Associated with Poor Prognosis

	von Stedingk K et al

	Transl Oncol 2013;6(4):447-57

			Application: IHC				
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																						DKC1 overexpression associated with prostate cancer progression

	Sieron P et al

	Br J Cancer 2009;101(8):1410-6

			
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
3.

												
																						Molecular markers implicating early malignant events in cervical carcinogenesis

	Koskimaa HM et al

	Cancer Epidemiol Biomarkers Prev 2010;19(8):2003-12

			
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
4.

												
																						Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells

	Stadler C et al

	Nat Methods 2013;10(4):315-23

			
											 
																						
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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