We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Antibody staining overlaps with GFP tagged protein
Figure description
Immunofluorescent staining of transgenic HeLa cells show antibody staining in nucleus & nucleoli and GFP expression in nucleoli.
Antibody dilution
1:60
Standard validation
Approved
Figure description
Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles.
Antibody dilution
1:50
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
WESTERN BLOT
Antibody HPA000167
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY419325)
Target mass (kDa)
52.3, 49.9, 49.1
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA000167
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
