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ANTIBODY INFORMATION
 
 

Antibody HPA010025

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA010025  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 3  

References

 3  

Proper citation

 Atlas Antibodies Cat#HPA010025, RRID:AB_1079900  

VALIDATION SUMMARY
 
 

Antibody HPA010025

  
Immunocytochemistry


Validated
  
Immunohistochemistry

Approved  
Western blot


Validated
  
Protein array

Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA010025

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by both siRNA:s.		  

 

Figure description

 Whole cells of segmented cells in 10x-images  

Antibody dilution

 1:102  
 

Standard validation

 Supported  

 

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to endoplasmic reticulum.  

Antibody dilution

 1:105  

Literature conformity

 The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA010025

  

Standard validation

 Approved  

 

Figure description

 Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 58 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:250  

Literature conformity

 Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA010025

  

Formal validation: Genetic
Validated

Downregulation visible in one of two siRNA lanes		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		  

Target mass (kDa)

 23.8, 21.2, 18.5  

Loading control

 Total protein image  

Antibody dilution

 1:228  
 

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

 

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 23.8, 21.2, 18.5  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA010025

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA010025

  

Antigen

 Recombinant protein fragment  

Length (aa)

 110  

Antigen sequence

 
EPDVVVKRQEALAAARLKMQEELNAQVEKHKEKLKQLEEEKRRQKIEMWD
SMQEGKSYKGNAKKPQEEDSPGPSTSSVLKRKSDRKPLRGGGYNPLSGEG
GGACSWRPGR
 

Matching transcripts

 VIMP-001 - ENSP00000381282 [100%]
VIMP-002 - ENSP00000433541 [100%]
VIMP-005 - ENSP00000433803 [100%]
VIMP-006 - ENSP00000434842 [98%]
 

Other gene match

  
ANTIGEN VIEW
VIMP-001
VIMP-002
VIMP-005
VIMP-006

RELEVANT PUBLICATIONS
 
 

Antibody HPA010025

  

1.

 The impact of tissue fixatives on morphology and antibody-based protein profiling in tissues and cells
Paavilainen L et al
J Histochem Cytochem 2010;58(3):237-46
  

2.

 Alternative transcripts and 3'UTR elements govern the incorporation of selenocysteine into selenoprotein S
Bubenik JL et al
PLoS One 2013;8(4):e62102
Application: ICC-IF, WB 		 

3.

 Selective up-regulation of human selenoproteins in response to oxidative stress
Touat-Hamici Z et al
J Biol Chem 2014;289(21):14750-61
Application: WB 		 
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.