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PCNA

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GENERAL INFORMATION

Gene name

 PCNA

Gene description

 Proliferating cell nuclear antigen

Protein class

 Cancer-related genes
Disease related genes
Plasma proteins
Predicted intracellular proteins

Predicted localization

 Intracellular

Number of transcripts

 2
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HUMAN PROTEIN ATLAS INFORMATION

Summary

 Localized to the nuclear bodies & nucleoplasm.

RNA cell category

 Expressed in all

Protein evidence

 Evidence at protein level

Main location

 Localized to the Nucleoplasm (supported), Nuclear bodies (supported)

Single-cell variation

 Single-cell variation in protein expression observed.

Cell cycle dependency

 Cell cycle dependent expression determined by custom assay

GFP

 The location(s) are Validated based on analysis of GFP-tagged protein.

DATA RELIABILITY

Reliability score

 Supported based on 2 antibodies.
CAB000148 , HPA030522
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ASSAYS
RNA expression
Human cells
Custom data
GFP

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all
Organ
Origin
Category
Expression
Alphabetical
Cell lines sorted after organ of phenotypic resemblance.

HUMAN CELLS

Summary

 Localized to the nuclear bodies & nucleoplasm.

Main location

 Nucleoplasm (supported), Nuclear bodies (supported)

 

 

 
Toggle channels
Low
High
G1
S
G2
M
N/A

Thumbnail

Antibody

Cell line

Location

Single-cell variation

Cell cycle dependent variation
CAB000148 A-431 Nucleoplasm
CAB000148 U-2 OS No staining    
CAB000148 U-251 MG No staining    
HPA030522 A-431 Nucleoplasm Intensity
HPA030522 U-2 OS Nuclear bodies Intensity Intensity
HPA030522 U-251 MG Nucleoplasm Intensity

CUSTOM DATA

 

 

 
Toggle channels

Thumbnail

Antibody

Cell line

Assay

Description
HPA030522 U-2 OS Image Staining of U-2 OS FUCCI cells, to characterize the cell cycle dependency of the protein expression pattern. The FUCCI cells, Fluorescence Ubiquitination Cell Cycle Indicator, are cells tagged with different fluorescent proteins fused to two different cell cycle regulators Cdt1 (expressed in G1 phase) and Geminin (expressed in S and G2 phases) that allows cell cycle monitoring. When both proteins are present, the overlay of the images appear in yellow marking the G1/S transition. PCNA is mostly expressed at the G1/S checkpoint, and during S and/or G2.

CELL CYCLE

Summary

 Expression has cell cycle dependency according to custom assay

Intensity variation

 Nuclear bodies, Nucleoplasm

GFP

Antibody

 HPA030522

Validation

C-terminal tag
BAC cell line: 5616
Validated: Antibody staining overlaps with GFP tagged protein but show additional locations

 

 

 
Toggle channels
Low
High
G1
S
G2
M
N/A

Thumbnail

Antibody

Cell line

BAC id

Tag

Location antibody staining

Location tagged protein
HPA030522 HeLa 5616 C-terminal Nucleus, Cytosol Nucleoplasm
HPA030522 HeLa Normal - Nucleus, Cytosol -

GENE INFORMATION

Gene name

 PCNA

Synonyms

Description

 Proliferating cell nuclear antigen (HGNC Symbol)

Entrez gene summary

 The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]

Chromosome

 20

Cytoband

 p12.3

Chromosome location (bp)

 5114953 - 5126626

Protein evidence

 Evidence at protein level (all genes)

Ensembl

 ENSG00000132646 (version 83.38)

Entrez gene

 5111

UniProt

 P12004 (UniProt - Evidence at protein level)

neXtProt

 NX_P12004

Antibodypedia

 PCNA antibodies

PROTEIN BROWSER
PCNA-001
PCNA-002

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)
PCNA-001
ENSP00000368438
ENST00000379143
P12004 [Direct mapping]
Proliferating cell nuclear antigen
Show all
Predicted intracellular proteins
Plasma proteins
Cancer-related genes
   Candidate cancer biomarkers
Disease related genes
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000082 [G1/S transition of mitotic cell cycle]
GO:0000083 [regulation of transcription involved in G1/S transition of mitotic cell cycle]
GO:0000278 [mitotic cell cycle]
GO:0000701 [purine-specific mismatch base pair DNA N-glycosylase activity]
GO:0000722 [telomere maintenance via recombination]
GO:0000723 [telomere maintenance]
GO:0000784 [nuclear chromosome, telomeric region]
GO:0003677 [DNA binding]
GO:0003682 [chromatin binding]
GO:0003684 [damaged DNA binding]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005663 [DNA replication factor C complex]
GO:0005737 [cytoplasm]
GO:0005813 [centrosome]
GO:0006271 [DNA strand elongation involved in DNA replication]
GO:0006272 [leading strand elongation]
GO:0006275 [regulation of DNA replication]
GO:0006281 [DNA repair]
GO:0006283 [transcription-coupled nucleotide-excision repair]
GO:0006284 [base-excision repair]
GO:0006289 [nucleotide-excision repair]
GO:0006297 [nucleotide-excision repair, DNA gap filling]
GO:0006298 [mismatch repair]
GO:0008283 [cell proliferation]
GO:0019985 [translesion synthesis]
GO:0030337 [DNA polymerase processivity factor activity]
GO:0030855 [epithelial cell differentiation]
GO:0030971 [receptor tyrosine kinase binding]
GO:0032077 [positive regulation of deoxyribonuclease activity]
GO:0032139 [dinucleotide insertion or deletion binding]
GO:0032201 [telomere maintenance via semi-conservative replication]
GO:0032405 [MutLalpha complex binding]
GO:0033683 [nucleotide-excision repair, DNA incision]
GO:0034644 [cellular response to UV]
GO:0035035 [histone acetyltransferase binding]
GO:0042276 [error-prone translesion synthesis]
GO:0042769 [DNA damage response, detection of DNA damage]
GO:0042802 [identical protein binding]
GO:0043596 [nuclear replication fork]
GO:0043626 [PCNA complex]
GO:0045739 [positive regulation of DNA repair]
GO:0045740 [positive regulation of DNA replication]
GO:0070062 [extracellular exosome]
GO:0070182 [DNA polymerase binding]
GO:0070557 [PCNA-p21 complex]
GO:0070911 [global genome nucleotide-excision repair]
GO:0070987 [error-free translesion synthesis]
Show all
 261 aa
28.8 kDa 		No 0
PCNA-002
ENSP00000368458
ENST00000379160
P12004 [Direct mapping]
Proliferating cell nuclear antigen
Show all
Predicted intracellular proteins
Plasma proteins
Cancer-related genes
   Candidate cancer biomarkers
Disease related genes
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000082 [G1/S transition of mitotic cell cycle]
GO:0000083 [regulation of transcription involved in G1/S transition of mitotic cell cycle]
GO:0000278 [mitotic cell cycle]
GO:0000701 [purine-specific mismatch base pair DNA N-glycosylase activity]
GO:0000722 [telomere maintenance via recombination]
GO:0000723 [telomere maintenance]
GO:0000784 [nuclear chromosome, telomeric region]
GO:0003677 [DNA binding]
GO:0003682 [chromatin binding]
GO:0003684 [damaged DNA binding]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005663 [DNA replication factor C complex]
GO:0005737 [cytoplasm]
GO:0005813 [centrosome]
GO:0006271 [DNA strand elongation involved in DNA replication]
GO:0006272 [leading strand elongation]
GO:0006275 [regulation of DNA replication]
GO:0006281 [DNA repair]
GO:0006283 [transcription-coupled nucleotide-excision repair]
GO:0006284 [base-excision repair]
GO:0006289 [nucleotide-excision repair]
GO:0006297 [nucleotide-excision repair, DNA gap filling]
GO:0006298 [mismatch repair]
GO:0007507 [heart development]
GO:0008283 [cell proliferation]
GO:0019985 [translesion synthesis]
GO:0030331 [estrogen receptor binding]
GO:0030337 [DNA polymerase processivity factor activity]
GO:0030855 [epithelial cell differentiation]
GO:0030971 [receptor tyrosine kinase binding]
GO:0032077 [positive regulation of deoxyribonuclease activity]
GO:0032139 [dinucleotide insertion or deletion binding]
GO:0032201 [telomere maintenance via semi-conservative replication]
GO:0032405 [MutLalpha complex binding]
GO:0033683 [nucleotide-excision repair, DNA incision]
GO:0033993 [response to lipid]
GO:0034644 [cellular response to UV]
GO:0035035 [histone acetyltransferase binding]
GO:0042276 [error-prone translesion synthesis]
GO:0042769 [DNA damage response, detection of DNA damage]
GO:0042802 [identical protein binding]
GO:0043596 [nuclear replication fork]
GO:0043626 [PCNA complex]
GO:0045739 [positive regulation of DNA repair]
GO:0045740 [positive regulation of DNA replication]
GO:0046686 [response to cadmium ion]
GO:0070062 [extracellular exosome]
GO:0070182 [DNA polymerase binding]
GO:0070557 [PCNA-p21 complex]
GO:0070911 [global genome nucleotide-excision repair]
GO:0070987 [error-free translesion synthesis]
Show all
 261 aa
28.8 kDa 		No 0

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.