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ANTIBODY INFORMATION VALIDATION SUMMARY IMMUNOCYTOCHEMISTRY IMMUNOHISTOCHEMISTRY WESTERN BLOT PROTEIN ARRAY ANTIGEN INFORMATION RELEVANT PUBLICATIONS

ANTIBODY INFORMATION

 
 

Antibody HPA049441

 

Antibody HPA050173

 

Antibody HPA072298

 

Provider

Atlas Antibodies
Sigma-Aldrich
  Atlas Antibodies
Sigma-Aldrich
  Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA049441   HPA050173   HPA072298  

Host species

Rabbit   Rabbit   Rabbit  

Clonality

pAb   pAb   pAb  

Purity

Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

     

Released in version

11   11   16  

References

     

Proper citation

Atlas Antibodies Cat#HPA049441, RRID:AB_2680768   Atlas Antibodies Cat#HPA050173, RRID:AB_2681042   Atlas Antibodies Cat#HPA072298, RRID:AB_2686510  

VALIDATION SUMMARY

 
 

Antibody HPA049441

 

Antibody HPA050173

 

Antibody HPA072298

 
Immunocytochemistry

    Approved  
Immunohistochemistry

Uncertain   Uncertain    
Western blot

Supported   Uncertain   Uncertain  
Protein array

Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY

 
 

Antibody HPA049441

 

Antibody HPA050173

 

Antibody HPA072298

 

Standard validation

    Approved  

     

Figure description

    Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.  

Antibody dilution

    1:22  

Literature conformity

    The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY

 
 

Antibody HPA049441

 

Antibody HPA050173

 

Antibody HPA072298

 

Standard validation

Uncertain   Uncertain    

     

Figure description

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.   Immunohistochemical staining of human testis shows cytoplasmic and nuclear positivity in cells in seminiferous ducts.    

Expression

RNA: detected in 35 tissues
Protein: detected in 68 cell types
  RNA: detected in 35 tissues
Protein: detected in 71 cell types
   

Retrieval

HIER pH6   HIER pH6    

Antibody dilution

1:80   1:80    

Literature conformity

No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.    

RNA consistency

Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.    

WESTERN BLOT

 
 

Antibody HPA049441

 

Antibody HPA050173

 

Antibody HPA072298

 

Standard validation

Supported

Band of predicted size in kDa (+/-20%) with additional bands present.
  Uncertain

Analysis performed using a standard panel of samples.
No bands detected.
  Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.
 

     

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY416625)
  Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
   

Target mass (kDa)

35.7   35.7   35.7  

Antibody dilution

1:250   1:530   1:80  

PROTEIN ARRAY

 
 

Antibody HPA049441

 

Antibody HPA050173

 

Antibody HPA072298

 

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.
  Supported

Pass with single peak corresponding to interaction only with its own antigen.
  Supported

Pass with single peak corresponding to interaction only with its own antigen.
 

     

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

1:4400   1:4200   1:600  

ANTIGEN INFORMATION

 
 

Antibody HPA049441

 

Antibody HPA050173

 

Antibody HPA072298

 

Antigen

Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

88   74   71  

Antigen sequence

CELGISKVELSMEDIETILNTLIYDGKVEMTIIAAKEGTVGSVDGHMKLY
RAVNPIIPPTGLVRAPCGLCPVFDDCHEGGEISPSNCI
 
VAINRLLSMGQLDLLRSNTGLLYRIKDSQNAGKMKGSDNQEKLVYQIIED
AGNKGIWSRDIRYKSNLPLTEINK
 
IKAVKSVAASKKKVYMLYNLQPDRSVTGGAWYSDQDFESEFVEVLNQQCF
KFLQSKAETARESKQNPMIQR
 

Matching transcripts

POLR3F-001 - ENSP00000366828 [100%]
  POLR3F-001 - ENSP00000366828 [100%]
  POLR3F-001 - ENSP00000366828 [100%]
 

Other gene match

     
ANTIGEN VIEW
POLR3F-001
 

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