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Immunofluorescent staining of human cell line A-431 shows localization to nucleus & cytosol.
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & plasma membrane.
Antibody dilution
1:100
1:100
Literature conformity
The subcellular location is not consistent with literature.
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA027394
Antibody HPA027399
Antibody HPA027402
Standard validation
Uncertain
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human stomach, lower shows moderate cytoplasmic and nucleolar positivity in glandular cells.
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction centra.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Expression
RNA: detected in 35 tissues Protein: detected in 57 cell types
RNA: detected in 35 tissues Protein: detected in 30 cell types
RNA: detected in 35 tissues Protein: detected in 36 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:200
1:600
1:150
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA027394
Antibody HPA027399
Antibody HPA027402
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402444)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402444)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402444)
Target mass (kDa)
31.7, 25.3
31.7
31.7, 25.3
Antibody dilution
1:250
1:250
1:125
PROTEIN ARRAY
Antibody HPA027394
Antibody HPA027399
Antibody HPA027402
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
