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Immunofluorescent staining of human cell line A-431 shows localization to nucleus.
Antibody dilution
1:29
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA008732
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 36 tissues Protein: detected in 55 cell types
Retrieval
HIER pH6
Antibody dilution
1:10
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA008732
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY420771)
Target mass (kDa)
60.3, 60.1, 59.1, 58.7, 58.4, 57.5, 57.4
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA008732
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:3000
ANTIGEN INFORMATION
Antibody HPA008732
Antigen
Recombinant protein fragment
Length (aa)
102
Antigen sequence
PDVEECAPNTGQCHLCLPPSEPWDQTLGVVNFPRKASRKPPREESSATCV
LEQPGALGAQILLVQRPNSGLLAGLWEFPSVTWEPSEQLQRKALLQELQR
WA
