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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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KRAS
  • TISSUE

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  • PATHOLOGY

PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

KRAS

Gene description

Kirsten rat sarcoma viral oncogene homolog

Protein class

Cancer-related genes
Disease related genes
Predicted intracellular proteins
RAS pathway related proteins

Predicted localization

Intracellular

Number of transcripts

4
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Expressed in all
HPA (cell line):Expressed in all
HPA (normal tissue):Expressed in all

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Cytoplasmic and membranous expression in several tissues.

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining mainly not consistent with RNA expression data. Caution, targets protein from more than one gene.

Reliability score

Approved based on 1 antibody.
HPA049830
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PROGNOSTIC SUMMARY

Prognostic marker in renal cancer (favourable) and pancreatic cancer (unfavourable).
Renal cancer p<0.001
Pancreatic cancer p<0.001
Show all

RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Liver cancer

STAINING SUMMARY

HPA049830

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Moderate to strong cytoplasmic and membranous immunoreactivity was displayed in several Hodgkin's lymphomas, colorectal and stomach cancers along with a few ovarian, endometrial, prostate, liver and pancreatic cancers. Remaining cancer cells were weakly stained or negative.

GENE INFORMATION

Gene name

KRAS (HGNC Symbol)

Synonyms

KRAS1, KRAS2

Description

Kirsten rat sarcoma viral oncogene homolog (HGNC Symbol)

Entrez gene summary

This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]

Chromosome

12

Cytoband

p12.1

Chromosome location (bp)

25204789 - 25250936

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000133703 (version 83.38)

Entrez gene

3845

UniProt

P01116 (UniProt - Evidence at protein level)

neXtProt

NX_P01116

Antibodypedia

KRAS antibodies

PROTEIN BROWSER

KRAS-001
KRAS-002
KRAS-003
KRAS-004

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

KRAS-001
ENSP00000308495
ENST00000311936
P01116 [Direct mapping]
GTPase KRas GTPase KRas, N-terminally processed
A0A024RAV5 [Target identity:100%; Query identity:100%]
V-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog, isoform CRA_b
Show all
Predicted intracellular proteins
RAS pathway related proteins
Cancer-related genes
   Candidate cancer biomarkers
   Mutated cancer genes
   Mutational cancer driver genes
   COSMIC somatic mutations in cancer genes
   COSMIC Somatic Mutations
   COSMIC Missense Mutations
Disease related genes
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000165 [MAPK cascade]
GO:0000186 [activation of MAPKK activity]
GO:0001934 [positive regulation of protein phosphorylation]
GO:0002223 [stimulatory C-type lectin receptor signaling pathway]
GO:0005515 [protein binding]
GO:0005525 [GTP binding]
GO:0005622 [intracellular]
GO:0005737 [cytoplasm]
GO:0005829 [cytosol]
GO:0005886 [plasma membrane]
GO:0005925 [focal adhesion]
GO:0007165 [signal transduction]
GO:0007173 [epidermal growth factor receptor signaling pathway]
GO:0007264 [small GTPase mediated signal transduction]
GO:0007265 [Ras protein signal transduction]
GO:0007411 [axon guidance]
GO:0007596 [blood coagulation]
GO:0008284 [positive regulation of cell proliferation]
GO:0008286 [insulin receptor signaling pathway]
GO:0008543 [fibroblast growth factor receptor signaling pathway]
GO:0010628 [positive regulation of gene expression]
GO:0015031 [protein transport]
GO:0016020 [membrane]
GO:0031234 [extrinsic component of cytoplasmic side of plasma membrane]
GO:0032403 [protein complex binding]
GO:0038095 [Fc-epsilon receptor signaling pathway]
GO:0045087 [innate immune response]
GO:0048010 [vascular endothelial growth factor receptor signaling pathway]
GO:0048011 [neurotrophin TRK receptor signaling pathway]
GO:0050900 [leukocyte migration]
Show all
188 aa
21.4 kDa
No 0
KRAS-002
ENSP00000451856
ENST00000556131
G3V4K2 [Direct mapping]
GTPase KRas
Show all
Predicted intracellular proteins
RAS pathway related proteins
Cancer-related genes
   Mutated cancer genes
   Mutational cancer driver genes
   COSMIC somatic mutations in cancer genes
   COSMIC Somatic Mutations
   COSMIC Missense Mutations
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005525 [GTP binding]
GO:0005622 [intracellular]
GO:0007165 [signal transduction]
GO:0007264 [small GTPase mediated signal transduction]
GO:0016020 [membrane]
Show all
43 aa
4.7 kDa
No 0
KRAS-003
ENSP00000452512
ENST00000557334
G3V5T7 [Direct mapping]
GTPase KRas
Show all
Predicted intracellular proteins
RAS pathway related proteins
Cancer-related genes
   Mutated cancer genes
   Mutational cancer driver genes
   COSMIC somatic mutations in cancer genes
   COSMIC Somatic Mutations
   COSMIC Missense Mutations
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005525 [GTP binding]
GO:0005622 [intracellular]
GO:0007165 [signal transduction]
GO:0007264 [small GTPase mediated signal transduction]
GO:0016020 [membrane]
Show all
75 aa
8.5 kDa
No 0
KRAS-004
ENSP00000256078
ENST00000256078
P01116 [Direct mapping]
GTPase KRas GTPase KRas, N-terminally processed
L7RSL8 [Target identity:100%; Query identity:100%]
V-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog; V-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog, isoform CRA_a
Show all
Predicted intracellular proteins
RAS pathway related proteins
Cancer-related genes
   Candidate cancer biomarkers
   Mutated cancer genes
   Mutational cancer driver genes
   COSMIC somatic mutations in cancer genes
   COSMIC Somatic Mutations
   COSMIC Missense Mutations
Disease related genes
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000165 [MAPK cascade]
GO:0000186 [activation of MAPKK activity]
GO:0001934 [positive regulation of protein phosphorylation]
GO:0002223 [stimulatory C-type lectin receptor signaling pathway]
GO:0003924 [GTPase activity]
GO:0005515 [protein binding]
GO:0005525 [GTP binding]
GO:0005622 [intracellular]
GO:0005737 [cytoplasm]
GO:0005739 [mitochondrion]
GO:0005829 [cytosol]
GO:0005886 [plasma membrane]
GO:0005925 [focal adhesion]
GO:0006886 [intracellular protein transport]
GO:0006913 [nucleocytoplasmic transport]
GO:0007165 [signal transduction]
GO:0007173 [epidermal growth factor receptor signaling pathway]
GO:0007264 [small GTPase mediated signal transduction]
GO:0007265 [Ras protein signal transduction]
GO:0007411 [axon guidance]
GO:0007596 [blood coagulation]
GO:0008284 [positive regulation of cell proliferation]
GO:0008286 [insulin receptor signaling pathway]
GO:0008542 [visual learning]
GO:0008543 [fibroblast growth factor receptor signaling pathway]
GO:0010628 [positive regulation of gene expression]
GO:0015031 [protein transport]
GO:0016020 [membrane]
GO:0019002 [GMP binding]
GO:0019003 [GDP binding]
GO:0019221 [cytokine-mediated signaling pathway]
GO:0021897 [forebrain astrocyte development]
GO:0030036 [actin cytoskeleton organization]
GO:0030275 [LRR domain binding]
GO:0031234 [extrinsic component of cytoplasmic side of plasma membrane]
GO:0032228 [regulation of synaptic transmission, GABAergic]
GO:0032403 [protein complex binding]
GO:0035022 [positive regulation of Rac protein signal transduction]
GO:0035176 [social behavior]
GO:0038002 [endocrine signaling]
GO:0038095 [Fc-epsilon receptor signaling pathway]
GO:0043406 [positive regulation of MAP kinase activity]
GO:0043524 [negative regulation of neuron apoptotic process]
GO:0045087 [innate immune response]
GO:0045121 [membrane raft]
GO:0045596 [negative regulation of cell differentiation]
GO:0048010 [vascular endothelial growth factor receptor signaling pathway]
GO:0048011 [neurotrophin TRK receptor signaling pathway]
GO:0048169 [regulation of long-term neuronal synaptic plasticity]
GO:0048873 [homeostasis of number of cells within a tissue]
GO:0050900 [leukocyte migration]
GO:0051000 [positive regulation of nitric-oxide synthase activity]
GO:0051092 [positive regulation of NF-kappaB transcription factor activity]
GO:0051146 [striated muscle cell differentiation]
GO:0051384 [response to glucocorticoid]
GO:0051385 [response to mineralocorticoid]
GO:0060441 [epithelial tube branching involved in lung morphogenesis]
Show all
189 aa
21.7 kDa
No 0

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