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TEX15

ANTIBODY INFORMATION
 
 

Antibody HPA036799

  

Antibody HPA036800

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA036799   HPA036800  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 12   7  

References

    

Proper citation

 Atlas Antibodies Cat#HPA036799, RRID:AB_2675321   Atlas Antibodies Cat#HPA036800, RRID:AB_10671714  

VALIDATION SUMMARY
 
 

Antibody HPA036799

  

Antibody HPA036800

  
Immunocytochemistry

Approved    
Immunohistochemistry

  Approved  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA036799

  

Antibody HPA036800

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles.    

Antibody dilution

 1:91    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA036799

  

Antibody HPA036800

  

Standard validation

   Approved  

   

Figure description

   Immunohistochemical staining of human testis shows moderate nuclear positivity in few cells in seminiferous ducts.  

Expression

   RNA: detected in 3 tissues
Protein: detected in 10 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:3000  

Literature conformity

   Consistent with gene/protein characterization data.  

RNA consistency

   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA036799

  

Antibody HPA036800

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 315.3   315.3  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA036799

  

Antibody HPA036800

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA036799

  

Antibody HPA036800

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 107   119  

Antigen sequence

 
STQNNETELTSPILLPDLQIKITNIFRPGFSPTADSLALKDSFCTHVTEA
TKPEINKEDGEILGFDIYSQPFGENADYPCEDKVDNIRQESGPVSNSEIS
LSFDLSR
  
SVYKRSMTEGSTVNTEYKNQKNQISEESCLNEKIITTNLIDSHLSTKNTT
TESVPLKNTVSNPLNKREKKGEIKVSKDSQSDLTLHSEIAYISKPGILGV
NHTPILPAHSETCKVPTLL
 

Matching transcripts

 TEX15-001 - ENSP00000256246 [100%]
  TEX15-001 - ENSP00000256246 [100%]
 

Other gene match

    
ANTIGEN VIEW
TEX15-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.