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LRP4

ANTIBODY INFORMATION
 
 

Antibody HPA011934

  

Antibody HPA012300

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA011934   HPA012300  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 4   4  

References

   1  

Proper citation

 Atlas Antibodies Cat#HPA011934, RRID:AB_1853286   Atlas Antibodies Cat#HPA012300, RRID:AB_1853288  

VALIDATION SUMMARY
 
 

Antibody HPA011934

  

Antibody HPA012300

  
Immunocytochemistry

Approved   Approved  
Immunohistochemistry

Approved   Approved  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA011934

  

Antibody HPA012300

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & mitochondria.   Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus, nucleoli & cytosol.  

Antibody dilution

 1:69   1:100  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA011934

  

Antibody HPA012300

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human pancreas shows cytoplasmic positivity.  

Expression

 RNA: detected in 29 tissues
Protein: detected in 64 cell types		   RNA: detected in 29 tissues
Protein: detected in 76 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:250   1:300  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA011934

  

Antibody HPA012300

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

    

Target mass (kDa)

 212   212  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA011934

  

Antibody HPA012300

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA011934

  

Antibody HPA012300

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 140   121  

Antigen sequence

 
IQRVDKYSGRNKETVLANVEGLMDIIVVSPQRQTGTNACGVNNGGCTHLC
FARASDFVCACPDEPDSQPCSLVPGLVPPAPRATGMSEKSPVLPNTPPTT
LYSSTTRTRTSLEEVEGRCSERDARLGLCARSNDAVPAAP
  
PGMGNLTYSNPSYRTSTQEVKIEAIPKPAMYNQLCYKKEGGPDHNYTKEK
IKIVEGICLLSGDDAEWDDLKQLRSSRGGLLRDHVCMKTDTVSIQASSGS
LDDTETEQLLQEEQSECSSVH
 

Matching transcripts

 LRP4-001 - ENSP00000367888 [99%]
  LRP4-001 - ENSP00000367888 [100%]
 

Other gene match

    
ANTIGEN VIEW
LRP4-001

RELEVANT PUBLICATIONS
 
 

Antibody HPA011934

  

Antibody HPA012300

  

1.

   Anti-LRP4 autoantibodies in AChR- and MuSK-antibody-negative myasthenia gravis
Pevzner A et al
J Neurol 2012;259(3):427-35
  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.