Cell atlas - YARS - The Human Protein Atlas

GENERAL INFORMATION
Gene name	YARS
Gene description	Tyrosyl-tRNA synthetase
Protein class	Disease related genes Enzymes Plasma proteins Potential drug targets Predicted intracellular proteins
Predicted localization	Intracellular
Number of transcripts	2
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HUMAN PROTEIN ATLAS INFORMATION
Summary	Mainly localized to the cytosol.
RNA cell category	Expressed in all
Protein evidence	Evidence at protein level
Main location	Localized to the Cytosol (validated)
siRNA	The location(s) are Validated based on siRNA mediated gene silencing.

DATA RELIABILITY
Reliability score	Validated based on 2 antibodies. HPA018950 , HPA018954
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ASSAYS

RNA expression

Human cells

siRNA

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all Organ Origin Category Expression Alphabetical Cell lines sorted after organ of phenotypic resemblance. Cell lines sorted after biological source for establishment. Cell lines sorted after the cell line category according to Cellosaurus. Cell lines sorted on descending RNA expression. Cell lines sorted alphabetically.

HUMAN CELLS

Summary

Mainly localized to the cytosol.

Main location

Cytosol (validated)


	Toggle channels	Low High G1 S G2 M N/A

Antibody	Cell line	Location
HPA018950	A-431	Nuclear bodies
HPA018950	A-431	Cytosol
HPA018950	U-2 OS	Nuclear bodies
HPA018950	U-2 OS	Cytosol
HPA018950	U-251 MG	Nuclear bodies
HPA018950	U-251 MG	Cytosol
HPA018954	A-431	Cytosol
HPA018954	U-2 OS	Cytosol
HPA018954	U-251 MG	Cytosol

siRNA VALIDATION

Antibody

HPA018954

siRNA validation

Validated: Significant downregulation > 25% by both siRNA:s.

Analysis performed in

Cytoplasmic region of segmented cells in 10x-images

siRNA 1

siRNA 2

RFI

49%

44%

p-value

< 0.01


	Toggle channels	Low High G1 S G2 M N/A

Antibody	Sample	Cell line
HPA018954	siRNA 1	U-2 OS
HPA018954	siRNA 2	U-2 OS
HPA018954	scrambled	U-2 OS

GENE INFORMATION
Gene name	YARS (HGNC Symbol)
Synonyms	tyrRS, YRS, YTS
Description	Tyrosyl-tRNA synthetase (HGNC Symbol)
Entrez gene summary	Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine. [provided by RefSeq, Jul 2008]
Chromosome	1
Cytoband	p35.1
Chromosome location (bp)	32775237 - 32818153
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000134684 (version 83.38)
Entrez gene	8565
UniProt	P54577 (UniProt - Evidence at protein level)
neXtProt	NX_P54577
Antibodypedia	YARS antibodies

PROTEIN BROWSER

YARS-001	YARS-201

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
YARS-001 ENSP00000362576 ENST00000373477	P54577 [Direct mapping] Tyrosine--tRNA ligase, cytoplasmic Tyrosine--tRNA ligase, cytoplasmic, N-terminally processed Show all	Enzymes ENZYME proteins Ligase Predicted intracellular proteins Plasma proteins Disease related genes Potential drug targets Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0000049 [tRNA binding] GO:0000166 [nucleotide binding] GO:0004812 [aminoacyl-tRNA ligase activity] GO:0004831 [tyrosine-tRNA ligase activity] GO:0004871 [signal transducer activity] GO:0005153 [interleukin-8 receptor binding] GO:0005524 [ATP binding] GO:0005615 [extracellular space] GO:0005634 [nucleus] GO:0005737 [cytoplasm] GO:0005829 [cytosol] GO:0006418 [tRNA aminoacylation for protein translation] GO:0006437 [tyrosyl-tRNA aminoacylation] GO:0006915 [apoptotic process] GO:0007165 [signal transduction] GO:0010467 [gene expression] GO:0044822 [poly(A) RNA binding] Show all	528 aa 59.1 kDa	No	0
YARS-201 ENSP00000484192 ENST00000616261	A0A0C4DGZ5 [Direct mapping] Tyrosine--tRNA ligase Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0000166 [nucleotide binding] GO:0004812 [aminoacyl-tRNA ligase activity] GO:0004831 [tyrosine-tRNA ligase activity] GO:0005524 [ATP binding] GO:0005737 [cytoplasm] GO:0005829 [cytosol] GO:0006418 [tRNA aminoacylation for protein translation] GO:0006437 [tyrosyl-tRNA aminoacylation] Show all	388 aa 43.9 kDa	No	0