HNF1A - Antibodies - The Human Protein Atlas

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HNF1A

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA035231

Antibody CAB010430

Provider

Atlas Antibodies
Sigma-Aldrich

Santa Cruz Biotechnology

Product name

HPA035231

sc-8986

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA035231, RRID:AB_2674528

Santa Cruz Biotechnology Cat#sc-8986, RRID:AB_2116746

VALIDATION SUMMARY

Antibody HPA035231

Antibody CAB010430

Immunocytochemistry

Approved

Immunohistochemistry

Western blot

Supported

Protein array

Approved

IMMUNOCYTOCHEMISTRY

Antibody HPA035231

Antibody CAB010430

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm.

Antibody dilution

1:80

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA035231

Antibody CAB010430

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.

Formal validation: Orthogonal

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.

Standard validation

Supported

Figure description

Immunohistochemical staining of human small intestine shows strong nuclear positivity in glandular cells.

Immunohistochemical staining of human small intestine shows nuclear positivity in glandular cells.

Expression

RNA: detected in 10 tissues
Protein: detected in 24 cell types

RNA: detected in 10 tissues
Protein: detected in 23 cell types

Retrieval

HIER pH6

Antibody dilution

1:250

1:60

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA035231

Antibody CAB010430

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 213, 118, 83, 50, 33, 26, 18
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

70.7, 68.1, 67.4, 58

70.7, 68.1, 67.4, 58, 48.5, 25.7, 23.8

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA035231

Antibody CAB010430

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA035231

Antibody CAB010430

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

Antigen sequence

VYNWFANRRKEEAFRHKLAMDTYSGPPPGPGPGPALPAHSSPGLPPPALS
PSKVHGVRYGQPATSETAEVPSSSGG

Matching transcripts

HNF1A-001 - ENSP00000257555 [100%]
HNF1A-002 - ENSP00000476181 [100%]
HNF1A-023 - ENSP00000443112 [100%]
HNF1A-025 - ENSP00000438804 [100%]

Other gene match

ANTIGEN VIEW

HNF1A-001	HNF1A-002	HNF1A-023	HNF1A-025

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.