CAPRIN1 - Antibodies - The Human Protein Atlas

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CAPRIN1

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA018126

Antibody HPA063617

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA018126

HPA063617

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA018126, RRID:AB_1849929

Atlas Antibodies Cat#HPA063617, RRID:AB_2685062

VALIDATION SUMMARY

Antibody HPA018126

Antibody HPA063617

Immunocytochemistry

Immunohistochemistry

Approved

Western blot

Uncertain

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA018126

Antibody HPA063617

Formal validation: Genetic

Validated

Significant downregulation > 25% by both siRNA:s.

Figure description

Cytoplasmic region of segmented cells in 10x-images

Antibody dilution

1:64

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.

Antibody dilution

1:50

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA018126

Antibody HPA063617

Standard validation

Approved

Figure description

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.

Immunohistochemical staining of human duodenum shows strong positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 79 cell types

RNA: detected in 37 tissues
Protein: detected in 80 cell types

Retrieval

HIER pH6

Antibody dilution

1:800

1:900

Literature conformity

Consistent with gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA018126

Antibody HPA063617

Formal validation: Genetic

Validated

Downregulation visible in both siRNA lanes

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled

Target mass (kDa)

78.4, 76.9, 72.8, 70.4

Loading control

Total protein image

Antibody dilution

1:142

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Figure description

Target mass (kDa)

78.4, 76.9, 72.8, 70.4

78.4, 76.9, 72.8, 70.4, 20.2

Antibody dilution

1:250

1:230

PROTEIN ARRAY

Antibody HPA018126

Antibody HPA063617

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:10000

1:2700

ANTIGEN INFORMATION

Antibody HPA018126

Antibody HPA063617

Antigen

Recombinant protein fragment

Length (aa)

136

Antigen sequence

LWDLLEGKEKPVCGTTYKVLKEIVERVFQSNYFDSTHNHQNGLCEEEEAA
SAPAVEDQVPEAEPEPAEEYTEQSEVESTEYVNRQFMAETQFTSGEKEQV
DEWTVETVEVVNSLQQQPQAASPSVPEPHSLTPVAQ

TIKKTARREQLMREEAEQKRLKTVLELQYVLDKLGDDEVRTDLKQGLNGV
PILSEEELSLLDEFYKLVDPERDM

Matching transcripts

CAPRIN1-001 - ENSP00000340329 [100%]
CAPRIN1-002 - ENSP00000431581 [100%]
CAPRIN1-009 - ENSP00000434150 [100%]
CAPRIN1-016 - ENSP00000374296 [100%]
CAPRIN1-017 - ENSP00000434204 [100%]
CAPRIN1-201 - ENSP00000483169 [100%]

Other gene match

ANTIGEN VIEW

CAPRIN1-001	CAPRIN1-002	CAPRIN1-007	CAPRIN1-009	CAPRIN1-016	CAPRIN1-017	CAPRIN1-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.