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ANTIBODY INFORMATION
 
 

Antibody HPA038602

  

Antibody HPA057592

  

Antibody CAB006329

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Abnova
  

Product name

 HPA038602   HPA057592   H00023479-M01  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 12   13   3  

References

      

Proper citation

 Atlas Antibodies Cat#HPA038602, RRID:AB_2676109   Atlas Antibodies Cat#HPA057592, RRID:AB_2683478   Abnova Corporation Cat#H00023479-M01, RRID:AB_425907  

VALIDATION SUMMARY
 
 

Antibody HPA038602

  

Antibody HPA057592

  

Antibody CAB006329

  
Immunocytochemistry

Supported      
Immunohistochemistry

Approved   Approved   Approved  
Western blot

Uncertain   Uncertain   Supported  
Protein array

Supported   Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA038602

  

Antibody HPA057592

  

Antibody CAB006329

  

Standard validation

 Supported      

     

Figure description

 Immunofluorescent staining of human cell line PC-3 shows localization to cytosol.      

Antibody dilution

 1:56      

Literature conformity

 The subcellular location is supported by literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA038602

  

Antibody HPA057592

  

Antibody CAB006329

  

Standard validation

 Approved   Approved   Approved  

     

Figure description

 Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.   Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 76 cell types		   RNA: detected in 37 tissues
Protein: detected in 37 cell types		   RNA: detected in 37 tissues
Protein: detected in 74 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:300   1:225   1:15  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA038602

  

Antibody HPA057592

  

Antibody CAB006329

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

     Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 18, 15.3   18, 16.9, 16.6, 15.3   18, 16.9, 16.6, 15.3  

Antibody dilution

 1:250   1:370   1:500  

PROTEIN ARRAY
 
 

Antibody HPA038602

  

Antibody HPA057592

  

Antibody CAB006329

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:4450    

ANTIGEN INFORMATION
 
 

Antibody HPA038602

  

Antibody HPA057592

  

Antibody CAB006329

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 69   61    

Antigen sequence

 
TFGCGSAIASSSLATEWVKGKTVEEALTIKNTDIAKELCLPPVKLHCSML
AEDAIKAALADYKLKQEPK
  
PARLYHKKVVDHYENPRNVGSLDKTSKNVGTGLVGAPACGDVMKLQIQVD
EKGKIVDARFK
  

											 
																				

																		

										
											
Matching transcripts

												
																					ISCU-001 - ENSP00000310623 [100%] 
ISCU-006 - ENSP00000376554 [100%] 

											 
																						ISCU-001 - ENSP00000310623 [100%] 
ISCU-003 - ENSP00000445598 [100%] 
ISCU-004 - ENSP00000411108 [100%] 
ISCU-007 - ENSP00000443272 [100%] 
ISCU-012 - ENSP00000446606 [100%] 
ISCU-006 - ENSP00000376554 [87%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ISCU-001
ISCU-003
ISCU-004
ISCU-006
ISCU-007
ISCU-012
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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