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Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Antibody dilution
1:112
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA040145
Antibody HPA059885
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA059885 across 73 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA040145 across 73 cell types.
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human adrenal gland shows moderate cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 66 cell types
RNA: detected in 37 tissues Protein: detected in 24 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:450
1:75
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA040145
Antibody HPA059885
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
146.6, 145.7, 139.6
146.6, 145.7, 139.6
Antibody dilution
1:250
1:140
PROTEIN ARRAY
Antibody HPA040145
Antibody HPA059885
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:6000
1:1700
ANTIGEN INFORMATION
Antibody HPA040145
Antibody HPA059885
Antigen
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
102
70
Antigen sequence
VTVTHKKAPSSLIDDCMEKFSLHEQQRLKIQGEQRGPDPGEDLADLEVVV
PGSPGDCLPEEADGTDTHLGLPAGASQPALTSSRVCFPERILEDSGFDEQ
QE
