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LMO7

ANTIBODY INFORMATION
 
 

Antibody HPA020923

  

Antibody HPA050184

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA020923   HPA050184  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 5   12  

References

 2    

Proper citation

 Atlas Antibodies Cat#HPA020923, RRID:AB_1852963   Atlas Antibodies Cat#HPA050184, RRID:AB_2681045  

VALIDATION SUMMARY
 
 

Antibody HPA020923

  

Antibody HPA050184

  
Immunocytochemistry

Approved   Approved  
Immunohistochemistry

Approved    
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA020923

  

Antibody HPA050184

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to cytosol & actin filaments.   Immunofluorescent staining of human cell line A-431 shows localization to actin filaments.  

Antibody dilution

 1:100   1:192  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA020923

  

Antibody HPA050184

  

Standard validation

 Approved    

   

Figure description

 Immunohistochemical staining of human stomach shows strong cytoplasmic and membranous positivity in glandular cells.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 44 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:150    

Literature conformity

 Partly consistent with gene/protein characterization data.    

RNA consistency

 Mainly not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA020923

  

Antibody HPA050184

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 190.7, 186.2, 160, 158.2, 153.7, 145.4, 121.4, 119.5, 23   190.7, 186.2, 160, 158.2, 153.7, 145.4, 121.4, 119.5, 34.4  

Antibody dilution

 1:250   1:960  

PROTEIN ARRAY
 
 

Antibody HPA020923

  

Antibody HPA050184

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:7000  

ANTIGEN INFORMATION
 
 

Antibody HPA020923

  

Antibody HPA050184

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 144   143  

Antigen sequence

 
KIYGENGSKSMSDVSAEDVQNLRQLRYEEMQKIKSQLKEQDQKWQDDLAK
WKDRRKSYTSDLQKKKEEREEIEKQALEKSKRSSKTFKEMLQDRESQNQK
STVPSRRRMYSFDDVLEEGKRPPTMTVSEASYQSERVEEKGATY
  
QLASSLSSQKEVAATEEDVTRLPSPTSPFSSLSQDQAATSKATLSSTSGL
DLMSESGEGEISPQREVSRSQDQFSDMRISINQTPGKSLDFGFTIKWDIP
GIFVASVEAGSPAEFSQLQVDDEIIAINNTKFSYNDSKEWEEA
 

Matching transcripts

 LMO7-001 - ENSP00000342112 [100%]
LMO7-002 - ENSP00000433352 [100%]
LMO7-003 - ENSP00000388955 [100%]
LMO7-004 - ENSP00000366719 [100%]
LMO7-005 - ENSP00000317802 [100%]
LMO7-201 - ENSP00000349571 [100%]
LMO7-202 - ENSP00000366757 [100%]
LMO7-012 - ENSP00000431129 [95%]
LMO7-025 - ENSP00000431896 [83%]
  LMO7-001 - ENSP00000342112 [100%]
LMO7-002 - ENSP00000433352 [100%]
LMO7-003 - ENSP00000388955 [100%]
LMO7-004 - ENSP00000366719 [100%]
LMO7-005 - ENSP00000317802 [100%]
LMO7-012 - ENSP00000431129 [100%]
LMO7-201 - ENSP00000349571 [100%]
LMO7-202 - ENSP00000366757 [100%]
LMO7-021 - ENSP00000433591 [88%]
 

Other gene match

    
ANTIGEN VIEW
LMO7-001
LMO7-002
LMO7-003
LMO7-004
LMO7-005
LMO7-012
LMO7-021
LMO7-025
LMO7-201
LMO7-202

RELEVANT PUBLICATIONS
 
 

Antibody HPA020923

  

Antibody HPA050184

  

1.

 The interactome of LIM domain proteins: the contributions of LIM domain proteins to heart failure and heart development
Li A et al
Proteomics 2012;12(2):203-25
    

2.

 The emerin-binding transcription factor Lmo7 is regulated by association with p130Cas at focal adhesions
Wozniak MA et al
PeerJ 2013;1:e134
Application: ICC-IF 		   
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.