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HUS1

GENERAL INFORMATION

Gene name

 HUS1

Gene description

 HUS1 checkpoint clamp component

Protein class

 Predicted intracellular proteins

Predicted localization

 Intracellular

Number of transcripts

 4
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HUMAN PROTEIN ATLAS INFORMATION

RNA category
TCGA (cancer tissue):Expressed in all
HPA (cell line):Expressed in all
HPA (normal tissue):Mixed

Protein evidence

 Evidence at protein level

Protein expression
normal tissue

 General cytoplasmic expression, additional nuclear expression in several tissues.

ANTIBODY IHC RELIABILITY

Data reliability
description

 Antibody staining mainly consistent with RNA expression data.

Reliability score

 Approved based on 1 antibody.
HPA026787
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PROGNOSTIC SUMMARY
Prognostic marker in cervical cancer (unfavourable) and liver cancer (unfavourable).
Cervical cancer p<0.001
Liver cancer p<0.001
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RNA EXPRESSION OVERVIEW
TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION
Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Liver cancer

STAINING SUMMARY
HPA026787

GENE INFORMATION

Gene name

 HUS1

Synonyms

Description

 HUS1 checkpoint clamp component (HGNC Symbol)

Entrez gene summary

 The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2011]

Chromosome

 7

Cytoband

 p12.3

Chromosome location (bp)

 47695730 - 47979581

Protein evidence

 Evidence at protein level (all genes)

Ensembl

 ENSG00000136273 (version 83.38)

Entrez gene

 3364

UniProt

 O60921 (UniProt - Evidence at protein level)

neXtProt

 NX_O60921

Antibodypedia

 HUS1 antibodies

PROTEIN BROWSER
HUS1-001
HUS1-004
HUS1-005
HUS1-011

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)
HUS1-001
ENSP00000258774
ENST00000258774
O60921 [Direct mapping]
Checkpoint protein HUS1
A4D2F2 [Target identity:100%; Query identity:100%]
HUS1 checkpoint homolog (S. pombe); HUS1 checkpoint homolog (S. pombe), isoform CRA_a; cDNA FLJ76255, highly similar to Homo sapiens HUS1 checkpoint homolog (S. pombe) (HUS1), mRNA
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Predicted intracellular proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
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GO:0000077 [DNA damage checkpoint]
GO:0000724 [double-strand break repair via homologous recombination]
GO:0001932 [regulation of protein phosphorylation]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005730 [nucleolus]
GO:0005737 [cytoplasm]
GO:0006260 [DNA replication]
GO:0006281 [DNA repair]
GO:0006289 [nucleotide-excision repair]
GO:0006302 [double-strand break repair]
GO:0006468 [protein phosphorylation]
GO:0006974 [cellular response to DNA damage stimulus]
GO:0007093 [mitotic cell cycle checkpoint]
GO:0008156 [negative regulation of DNA replication]
GO:0009411 [response to UV]
GO:0009790 [embryo development]
GO:0030896 [checkpoint clamp complex]
GO:0031573 [intra-S DNA damage checkpoint]
GO:0033314 [mitotic DNA replication checkpoint]
GO:0035861 [site of double-strand break]
GO:0044778 [meiotic DNA integrity checkpoint]
GO:0071479 [cellular response to ionizing radiation]
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 280 aa
31.7 kDa 		No 0
HUS1-004
ENSP00000398806
ENST00000446009
C9JA95 [Direct mapping]
Checkpoint protein HUS1
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Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000077 [DNA damage checkpoint]
GO:0000724 [double-strand break repair via homologous recombination]
GO:0005730 [nucleolus]
GO:0006281 [DNA repair]
GO:0006289 [nucleotide-excision repair]
GO:0030896 [checkpoint clamp complex]
GO:0031573 [intra-S DNA damage checkpoint]
GO:0033314 [mitotic DNA replication checkpoint]
GO:0035861 [site of double-strand break]
GO:0044778 [meiotic DNA integrity checkpoint]
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 145 aa
16.4 kDa 		No 0
HUS1-005
ENSP00000416588
ENST00000432325
O60921 [Direct mapping]
Checkpoint protein HUS1
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Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000077 [DNA damage checkpoint]
GO:0000724 [double-strand break repair via homologous recombination]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005730 [nucleolus]
GO:0005737 [cytoplasm]
GO:0006260 [DNA replication]
GO:0006281 [DNA repair]
GO:0006289 [nucleotide-excision repair]
GO:0006302 [double-strand break repair]
GO:0006974 [cellular response to DNA damage stimulus]
GO:0030896 [checkpoint clamp complex]
GO:0031573 [intra-S DNA damage checkpoint]
GO:0033314 [mitotic DNA replication checkpoint]
GO:0035861 [site of double-strand break]
GO:0044778 [meiotic DNA integrity checkpoint]
GO:0071479 [cellular response to ionizing radiation]
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 259 aa
29.3 kDa 		No 0
HUS1-011
ENSP00000404855
ENST00000432627
C9JCK8 [Direct mapping]
Checkpoint protein HUS1
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Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000077 [DNA damage checkpoint]
GO:0000724 [double-strand break repair via homologous recombination]
GO:0005730 [nucleolus]
GO:0006281 [DNA repair]
GO:0006289 [nucleotide-excision repair]
GO:0030896 [checkpoint clamp complex]
GO:0031573 [intra-S DNA damage checkpoint]
GO:0033314 [mitotic DNA replication checkpoint]
GO:0035861 [site of double-strand break]
GO:0044778 [meiotic DNA integrity checkpoint]
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 183 aa
20.7 kDa 		No 0

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.