Cell atlas - WDR33 - The Human Protein Atlas

GENERAL INFORMATION
Gene name	WDR33
Gene description	WD repeat domain 33
Protein class	Predicted intracellular proteins Predicted membrane proteins
Predicted localization	Intracellular,Membrane
Number of transcripts	5
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HUMAN PROTEIN ATLAS INFORMATION
Summary	Mainly localized to the nucleoplasm. In addition localized to the nucleoli (fibrillar center).
RNA cell category	Expressed in all
Protein evidence	Evidence at protein level
Main location	Localized to the Nucleoplasm (supported)
Additional location	In addition localized to the Nucleoli fibrillar center (supported)
siRNA	The location(s) are Validated based on siRNA mediated gene silencing.
Mouse	Localized to the Nucleus, Cytosol in mouse cells.

DATA RELIABILITY
Reliability score	Supported based on 1 antibody. HPA046527
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ASSAYS

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all Organ Origin Category Expression Alphabetical Cell lines sorted after organ of phenotypic resemblance. Cell lines sorted after biological source for establishment. Cell lines sorted after the cell line category according to Cellosaurus. Cell lines sorted on descending RNA expression. Cell lines sorted alphabetically.

HUMAN CELLS

Summary

Mainly localized to the nucleoplasm. In addition localized to the nucleoli (fibrillar center).

Main location

Nucleoplasm (supported)

Additional location

Nucleoli fibrillar center (supported)


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Antibody	Cell line	Location
HPA046527	PC-3	Nucleoplasm
HPA046527	PC-3	Nucleoli fibrillar center
HPA046527	U-2 OS	Nucleoplasm
HPA046527	U-251 MG	Nucleoplasm
HPA046527	U-251 MG	Nucleoli fibrillar center

siRNA VALIDATION

Antibody

HPA046527

siRNA validation

Validated: Significant downregulation > 25% by one siRNA.

Analysis performed in

Nuclear region of segmented cells in 10x-images

siRNA 1

RFI

72%

p-value

< 0.01


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Thumbnail 10x	Thumbnail 40x	Antibody	Sample	Cell line
		HPA046527	siRNA 1	U-2 OS
		HPA046527	scrambled	U-2 OS

MOUSE CELLS

Main location

Nucleus, Cytosol

Mouse gene

ENSMUSG00000024400 (version 83)


	Toggle channels	Low High G1 S G2 M N/A

Thumbnail	Antibody	Cell line	Location
	HPA046527	NIH 3T3	Nucleus
	HPA046527	NIH 3T3	Cytosol

GENE INFORMATION
Gene name	WDR33 (HGNC Symbol)
Synonyms	FLJ11294, NET14, WDC146
Description	WD repeat domain 33 (HGNC Symbol)
Entrez gene summary	This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene is highly expressed in testis and the protein is localized to the nucleus. This gene may play important roles in the mechanisms of cytodifferentiation and/or DNA recombination. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Chromosome	2
Cytoband	q14.3
Chromosome location (bp)	127701022 - 127811187
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000136709 (version 83.38)
Entrez gene	55339
UniProt	Q9C0J8 (UniProt - Evidence at protein level)
neXtProt	NX_Q9C0J8
Antibodypedia	WDR33 antibodies

PROTEIN BROWSER

WDR33-001	WDR33-002	WDR33-003	WDR33-004	WDR33-005

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
WDR33-001 ENSP00000325377 ENST00000322313	Q9C0J8 [Direct mapping] pre-mRNA 3' end processing protein WDR33 Show all	Predicted intracellular proteins Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0005515 [protein binding] GO:0005581 [collagen trimer] GO:0005634 [nucleus] GO:0005654 [nucleoplasm] GO:0005730 [nucleolus] GO:0005847 [mRNA cleavage and polyadenylation specificity factor complex] GO:0006301 [postreplication repair] GO:0006378 [mRNA polyadenylation] GO:0006379 [mRNA cleavage] GO:0007283 [spermatogenesis] GO:0044822 [poly(A) RNA binding] Show all	1336 aa 145.9 kDa	No	0
WDR33-002 ENSP00000387186 ENST00000409658	Q9C0J8 [Direct mapping] pre-mRNA 3' end processing protein WDR33 Show all	Predicted membrane proteins Prediction method-based Membrane proteins predicted by MDM MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins Phobius predicted membrane proteins SCAMPI predicted membrane proteins SPOCTOPUS predicted membrane proteins THUMBUP predicted membrane proteins TMHMM predicted membrane proteins # TM segments-based 1TM proteins predicted by MDM Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0005515 [protein binding] GO:0005581 [collagen trimer] GO:0005634 [nucleus] GO:0005654 [nucleoplasm] GO:0005730 [nucleolus] GO:0005847 [mRNA cleavage and polyadenylation specificity factor complex] GO:0006301 [postreplication repair] GO:0006378 [mRNA polyadenylation] GO:0006379 [mRNA cleavage] GO:0007283 [spermatogenesis] GO:0044822 [poly(A) RNA binding] Show all	326 aa 38.3 kDa	No	1
WDR33-003 ENSP00000376730 ENST00000393006	Q9C0J8 [Direct mapping] pre-mRNA 3' end processing protein WDR33 Show all	Predicted intracellular proteins Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0005515 [protein binding] GO:0005581 [collagen trimer] GO:0005634 [nucleus] GO:0005654 [nucleoplasm] GO:0005730 [nucleolus] GO:0005847 [mRNA cleavage and polyadenylation specificity factor complex] GO:0006301 [postreplication repair] GO:0006378 [mRNA polyadenylation] GO:0006379 [mRNA cleavage] GO:0007283 [spermatogenesis] GO:0044822 [poly(A) RNA binding] Show all	257 aa 30.3 kDa	No	0
WDR33-004 ENSP00000397547 ENST00000436787	C9J8B4 [Direct mapping] pre-mRNA 3' end-processing protein WDR33 Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0005515 [protein binding] GO:0005847 [mRNA cleavage and polyadenylation specificity factor complex] GO:0006378 [mRNA polyadenylation] GO:0006379 [mRNA cleavage] Show all	205 aa 23.2 kDa	No	0
WDR33-005 ENSP00000386861 ENST00000408998	B9A053 [Direct mapping] pre-mRNA 3' end-processing protein WDR33 Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0005847 [mRNA cleavage and polyadenylation specificity factor complex] GO:0006378 [mRNA polyadenylation] GO:0006379 [mRNA cleavage] Show all	118 aa 14 kDa	No	0