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ALDOB

ANTIBODY INFORMATION
 
 

Antibody HPA002198

  

Antibody CAB020827

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Origene
  

Product name

 HPA002198   3290.00.02  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 3   5  

References

 2    

Proper citation

 Atlas Antibodies Cat#HPA002198, RRID:AB_1078129    

VALIDATION SUMMARY
 
 

Antibody HPA002198

  

Antibody CAB020827

  
Immunohistochemistry


Validated
   Supported  
Western blot

Supported   Supported  
Protein array

Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA002198

  

Antibody CAB020827

  

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		    

   

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.    
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.   Immunohistochemical staining of human kidney shows strong positivity in proximal tubules.  

Expression

 RNA: detected in 19 tissues
Protein: detected in 5 cell types		   RNA: detected in 19 tissues
Protein: detected in 27 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:250   1:3000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA002198

  

Antibody CAB020827

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 39.5   39.5, 34.9  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA002198

  

Antibody CAB020827

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA002198

  

Antibody CAB020827

  

Antigen

 Recombinant protein fragment   Genetic immunization  

Length (aa)

 123    

Antigen sequence

 
KPNMVTAGHACTKKYTPEQVAMATVTALHRTVPAAVPGICFLSGGMSEED
ATLNLNAINLCPLPKPWKLSFSYGRALQASALAAWGGKAANKEATQEAFM
KRAMANCQAAKGQYVHTGSSGAA
  

											 
																				

																		

										
											
Matching transcripts

												
																					ALDOB-001 - ENSP00000363988 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ALDOB-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA002198

												
										 
																				
											
											
											
Antibody CAB020827

												
										 
																		

																	

										
											
1.

												
																						Aldolase directly interacts with ARNO and modulates cell morphology and acidic vesicle distribution

	Merkulova M et al

	Am J Physiol Cell Physiol 2011;300(6):C1442-55

			
											 
																						
											 
																				

																		

										
											
2.

												
																						The human liver-specific proteome defined by transcriptomics and antibody-based profiling

	Kampf C et al

	FASEB J 2014;28(7):2901-14

			
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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