Fields »
ABOUT
HELP
BLOG
POLR1E

ANTIBODY INFORMATION
 
 

Antibody HPA022527

  

Antibody HPA052400

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA022527   HPA052400  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 5   13  

References

 1    

Proper citation

 Atlas Antibodies Cat#HPA022527, RRID:AB_1854924   Atlas Antibodies Cat#HPA052400, RRID:AB_2681815  

VALIDATION SUMMARY
 
 

Antibody HPA022527

  

Antibody HPA052400

  
Immunocytochemistry

  Supported  
Immunohistochemistry

Supported    
Western blot

Supported   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA022527

  

Antibody HPA052400

  

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line HeLa shows localization to nucleus & nucleoli fibrillar center.  

Antibody dilution

   1:139  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA022527

  

Antibody HPA052400

  

Standard validation

 Supported    

   

Figure description

 Immunohistochemical staining of human rectum shows nucleolar and cytoplasmic positivity in glandular cells.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 62 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:85    

Literature conformity

 Consistent with extensive gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA022527

  

Antibody HPA052400

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 54   54, 47.3  

Antibody dilution

 1:250   1:690  

PROTEIN ARRAY
 
 

Antibody HPA022527

  

Antibody HPA052400

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:5500  

ANTIGEN INFORMATION
 
 

Antibody HPA022527

  

Antibody HPA052400

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 100   101  

Antigen sequence

 
LLQFPLGQDPSFLAIPILALPPSDSLVPPYIVWYIVWPSALISFLGCTLT
VQFSNGKLQSPGNMRFTLYENKDSTNPRKRNQRILAAETDRLSYVGNNFG
  
LPPCYDDAAKPEDVYKFEDLLSPAEYEALQSPSEAFRNVTSEEILKMIEE
NSHCTFVIEALKSLPSDVESRDRQARCIWFLDTLIKFRAHRVVKRKSALG
P
 

Matching transcripts

 POLR1E-002 - ENSP00000367023 [99%]
  POLR1E-001 - ENSP00000367029 [100%]
POLR1E-002 - ENSP00000367023 [100%]
 

Other gene match

    
ANTIGEN VIEW
POLR1E-001
POLR1E-002

RELEVANT PUBLICATIONS
 
 

Antibody HPA022527

  

Antibody HPA052400

  

1.

 Tumour expression of bladder cancer-associated urinary proteins
Lindén M et al
BJU Int 2013;112(3):407-15
    
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.