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Immunofluorescent staining of human cell line A-431 shows localization to nucleus & mitochondria.
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & mitochondria.
Antibody dilution
1:70
1:9
Literature conformity
The subcellular location is not consistent with literature.
The subcellular location is not consistent with literature.
WESTERN BLOT
Antibody HPA060231
Antibody HPA071546
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
89.8
89.8
Antibody dilution
1:230
1:80
PROTEIN ARRAY
Antibody HPA060231
Antibody HPA071546
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
