BUD13 - Antibodies - The Human Protein Atlas

We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.

BUD13

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA038340

Antibody HPA038341

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA038340

HPA038341

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA038340, RRID:AB_2675967

Atlas Antibodies Cat#HPA038341, RRID:AB_10671528

VALIDATION SUMMARY

Antibody HPA038340

Antibody HPA038341

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA038340

Antibody HPA038341

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA038341.

Validated

Antibody staining overlaps with antibody HPA038340.

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to nucleus.

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.

Antibody dilution

1:76

1:135

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA038340

Antibody HPA038341

Standard validation

Approved

Figure description

Immunohistochemical staining of human testis shows distinct nuclear positivity in cells in seminiferous ducts.

Expression

RNA: detected in 37 tissues
Protein: detected in 70 cell types

RNA: detected in 37 tissues
Protein: detected in 76 cell types

Retrieval

HIER pH6

Antibody dilution

1:60

1:50

Literature conformity

No avaliable gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA038340

Antibody HPA038341

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

70.5

70.5, 55.6

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA038340

Antibody HPA038341

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:12000

1:6000

ANTIGEN INFORMATION

Antibody HPA038340

Antibody HPA038341

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

HDSPDLAPNVTYSLPRTKSGKAPERASSKTSPHWKESGASHLSFPKNSKY
EYDPDISPPRKKQAKSHFGDKKQLDSKGDCQKAT

PGHQDSDSDLSPPRNRPRHRSSDSDLSPPRRRQRTKSSDSDLSPPRRSQP
PGKKAAHMYSGAKTGLVLTDIQREQQELKEQDQETM

Matching transcripts

BUD13-001 - ENSP00000260210 [100%]

BUD13-001 - ENSP00000260210 [100%]
BUD13-002 - ENSP00000364594 [100%]

Other gene match

ANTIGEN VIEW

BUD13-001	BUD13-002

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.