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Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.
Antibody dilution
1:83
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA038939
Antibody HPA038940
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in renal tubules.
Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
Expression
RNA: detected in 34 tissues Protein: detected in 35 cell types
RNA: detected in 34 tissues Protein: detected in 21 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:100
1:500
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA038939
Antibody HPA038940
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
48.9, 46.4
48.9, 46.4, 38.3
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA038939
Antibody HPA038940
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
