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CYP2C8

ANTIBODY INFORMATION
 
 

Antibody HPA013547

  

Antibody HPA013970

  

Antibody HPA015066

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA013547   HPA013970   HPA015066  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

     CYP2C9 - ENSG00000138109 [82%]
CYP2C19 - ENSG00000165841 [100%]
  

Released in version

 4   4   4  

References

      

Proper citation

 Atlas Antibodies Cat#HPA013547, RRID:AB_1847435   Atlas Antibodies Cat#HPA013970, RRID:AB_1847434   Atlas Antibodies Cat#HPA015066, RRID:AB_1847433  

VALIDATION SUMMARY
 
 

Antibody HPA013547

  

Antibody HPA013970

  

Antibody HPA015066

  
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA013547

  

Antibody HPA013970

  

Antibody HPA015066

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.  

Expression

 RNA: detected in 8 tissues
Protein: detected in 30 cell types		   RNA: detected in 8 tissues
Protein: detected in 20 cell types		   RNA: detected in 8 tissues
Protein: detected in 12 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:7   1:50   1:150  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA013547

  

Antibody HPA013970

  

Antibody HPA015066

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 55.8, 47.8, 44.4   55.8, 47.8, 44.4, 35.3   55.9, 55.8, 55.6, 47.8, 44.4, 35.3  

Antibody dilution

 1:250   1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA013547

  

Antibody HPA013970

  

Antibody HPA015066

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA013547

  

Antibody HPA013970

  

Antibody HPA015066

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 59   121   56  

Antigen sequence

 
KRICAGEGLARMELFLFLTTILQNFNLKSVDDLKNLNTTAVTKGIVSLPP
SYQICFIPV
  
ICSVVFQKRFDYKDQNFLTLMKRFNENFRILNSPWIQVCNNFPLLIDCFP
GTHNKVLKNVALTRSYIREKVKEHQASLDVNNPRDFIDCFLIKMEQEKDN
QKSEFNIENLVGTVADLFVAG
  
KEHQESMDINNPRDFIDCFLIKMEKEKQNQQSEFTIENLVITAADLLGAG
TETTST
 

Matching transcripts

 CYP2C8-001 - ENSP00000360317 [100%]
CYP2C8-201 - ENSP00000445062 [100%]
CYP2C8-202 - ENSP00000442343 [100%]
CYP2C8-203 - ENSP00000485110 [100%]
  CYP2C8-001 - ENSP00000360317 [100%]
CYP2C8-201 - ENSP00000445062 [100%]
CYP2C8-202 - ENSP00000442343 [100%]
CYP2C8-203 - ENSP00000485110 [100%]
CYP2C8-204 - ENSP00000487145 [100%]
  CYP2C8-001 - ENSP00000360317 [80%]
CYP2C8-201 - ENSP00000445062 [80%]
CYP2C8-202 - ENSP00000442343 [80%]
CYP2C8-203 - ENSP00000485110 [80%]
CYP2C8-204 - ENSP00000487145 [80%]
 

Other gene match

     CYP2C9 - ENSG00000138109 [82%]
CYP2C19 - ENSG00000165841 [100%]
  
ANTIGEN VIEW
CYP2C8-001
CYP2C8-201
CYP2C8-202
CYP2C8-203
CYP2C8-204
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.