IDH1 - Antibodies - The Human Protein Atlas

We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.

IDH1

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA035248

Antibody HPA057936

Antibody CAB033218

Antibody CAB062556

Provider

Atlas Antibodies
Sigma-Aldrich

Santa Cruz Biotechnology

Atlas Antibodies
Sigma-Aldrich

Product name

HPA035248

HPA057936

sc-49996

AMAb90578

Host species

Rabbit

Goat

Mouse

Clonality

pAb

msAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Affinity

Protein A/G

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA035248, RRID:AB_10600714

Atlas Antibodies Cat#HPA057936, RRID:AB_2683559

Santa Cruz Biotechnology Cat#sc-49996, RRID:AB_2123166

Atlas Antibodies Cat#AMAb90578, RRID:AB_2665593

VALIDATION SUMMARY

Antibody HPA035248

Antibody HPA057936

Antibody CAB033218

Antibody CAB062556

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA035248

Antibody HPA057936

Antibody CAB033218

Antibody CAB062556

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to nuclear bodies & cytosol.

Antibody dilution

1:2000

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA035248

Antibody HPA057936

Antibody CAB033218

Antibody CAB062556

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Formal validation: Orthogonal

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA057936 across 46 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA035248 across 46 cell lines.

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA057936 across 74 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA035248 across 74 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human prostate shows strong cytoplasmic and nuclear positivity in glandular cells.

Immunohistochemical staining of human prostate shows moderate cytoplasmic positivity in glandular cells.

Immunohistochemical staining of human prostate shows strong cytoplasmic and nuclear positivity in glandular cells.

Immunohistochemical staining of human prostate shows moderate nuclear, cytoplasmic and membranous positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 39 cell types

RNA: detected in 37 tissues
Protein: detected in 31 cell types

RNA: detected in 37 tissues
Protein: detected in 50 cell types

RNA: detected in 37 tissues
Protein: detected in 15 cell types

Retrieval

HIER pH6

Antibody dilution

1:250

1:75

1:10000

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA035248

Antibody HPA057936

Antibody CAB033218

Antibody CAB062556

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

46.7

46.7, 17.7, 8.3, 7.1

Antibody dilution

1:250

1:160

1:500

PROTEIN ARRAY

Antibody HPA035248

Antibody HPA057936

Antibody CAB033218

Antibody CAB062556

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

1:1900

ANTIGEN INFORMATION

Antibody HPA035248

Antibody HPA057936

Antibody CAB033218

Antibody CAB062556

Antigen

Recombinant protein fragment

Synthetic peptide

Recombinant protein

Length (aa)

Antigen sequence

FVVPGPGKVEITYTPSDGTQKVTYLVHNFEEGGGVAMGMYNQDKSIEDFA
HSSFQMALSKGWPLY

LAHRAKLDNNKELAFFANALEEVSIETIEAGFMTKDLAACIKGLPNVQRS
DYLNTFEFMDKLGENLKIKLAQA

Matching transcripts

IDH1-001 - ENSP00000260985 [100%]
IDH1-004 - ENSP00000410513 [100%]
IDH1-006 - ENSP00000390265 [100%]

Other gene match

ANTIGEN VIEW

IDH1-001	IDH1-004	IDH1-006

RELEVANT PUBLICATIONS

Antibody HPA035248

Antibody HPA057936

Antibody CAB033218

Antibody CAB062556

The proteomics of colorectal cancer: identification of a protein signature associated with prognosis
O'Dwyer D et al
PLoS One 2011;6(11):e27718

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.