VWA9 - Antibodies - The Human Protein Atlas

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VWA9

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA040255

Antibody HPA040651

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA040255

HPA040651

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA040255, RRID:AB_10805710

Atlas Antibodies Cat#HPA040651, RRID:AB_10796559

VALIDATION SUMMARY

Antibody HPA040255

Antibody HPA040651

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA040255

Antibody HPA040651

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, nuclear bodies & mitochondria.

Antibody dilution

1:108

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA040255

Antibody HPA040651

Standard validation

Approved

Figure description

Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferus ducts and leydig cells.

Immunohistochemical staining of human stomach, lower shows strong nuclear positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 57 cell types

RNA: detected in 37 tissues
Protein: detected in 56 cell types

Retrieval

HIER pH6

Antibody dilution

1:1200

1:120

Literature conformity

No avaliable gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA040255

Antibody HPA040651

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

61.3, 57.5, 53.6, 51.6, 50.7, 48.4

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA040255

Antibody HPA040651

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:30000

1:3000

ANTIGEN INFORMATION

Antibody HPA040255

Antibody HPA040651

Antigen

Recombinant protein fragment

Length (aa)

108

101

Antigen sequence

LTADVQVFPRPEPFVVDEEIDPIPKVINTDLEIVGFIDIADISSPPVLSR
HLVLPIALNKEGDEVGTGITDDNEDENSANQIAGKIPNFCVLLHGSLKVE
GMVAIVQL

GCLGIGRGSLRHSLATQNQRSESNRFPLPFPFPSKLYIMCMANLEELQST
DSLECLERLIDLNNGEGQIFTIDGPLCLKNVQSMFGKLIDLAYTPFHAVL
K

Matching transcripts

VWA9-002 - ENSP00000457624 [100%]
VWA9-003 - ENSP00000396314 [100%]
VWA9-004 - ENSP00000408429 [100%]
VWA9-006 - ENSP00000402898 [100%]
VWA9-008 - ENSP00000326379 [100%]
VWA9-007 - ENSP00000456460 [99%]

VWA9-002 - ENSP00000457624 [100%]
VWA9-003 - ENSP00000396314 [100%]
VWA9-004 - ENSP00000408429 [100%]
VWA9-006 - ENSP00000402898 [100%]
VWA9-007 - ENSP00000456460 [100%]
VWA9-008 - ENSP00000326379 [100%]

Other gene match

ANTIGEN VIEW

VWA9-002	VWA9-003	VWA9-004	VWA9-006	VWA9-007	VWA9-008

RELEVANT PUBLICATIONS

Antibody HPA040255

Antibody HPA040651

Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C et al
Nat Methods 2013;10(4):315-23

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.