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COL2A1
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ANTIBODY INFORMATION VALIDATION SUMMARY IMMUNOCYTOCHEMISTRY IMMUNOHISTOCHEMISTRY WESTERN BLOT PROTEIN ARRAY ANTIGEN INFORMATION RELEVANT PUBLICATIONS

ANTIBODY INFORMATION

 
 

Antibody HPA055753

 

Antibody CAB002214

 

Provider

Atlas Antibodies
Sigma-Aldrich
  Novocastra
 

Product name

HPA055753   NCL-COLL-IIp  

Host species

Rabbit   Rabbit  

Clonality

pAb   pAb  

Purity

Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

   

Released in version

16   1  

References

   

Proper citation

Atlas Antibodies Cat#HPA055753, RRID:AB_2682908    

VALIDATION SUMMARY

 
 

Antibody HPA055753

 

Antibody CAB002214

 
Immunocytochemistry

Approved    
Immunohistochemistry

  Supported  
Western blot

Supported   Uncertain  
Protein array

Approved    

IMMUNOCYTOCHEMISTRY

 
 

Antibody HPA055753

 

Antibody CAB002214

 

Standard validation

Approved    

   

Figure description

Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.    

Antibody dilution

1:66    

Literature conformity

The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY

 
 

Antibody HPA055753

 

Antibody CAB002214

 

Standard validation

  Supported  

   

Figure description

  Immunohistochemical staining of human nasopharynx shows moderate cytoplasmic positivity in chondrocytes.  

Expression

  RNA: detected in 4 tissues
Protein: detected in 11 cell types
 

Retrieval

  None  

Antibody dilution

  1:50  

Literature conformity

  Consistent with extensive gene/protein characterization data.  

RNA consistency

  Not consistent with RNA expression data.  

WESTERN BLOT

 
 

Antibody HPA055753

 

Antibody CAB002214

 

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.
  Uncertain

Analysis performed using a standard panel of samples.
Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
 

   

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
  Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
 

Target mass (kDa)

141.8, 134.4   141.8, 134.4  

Antibody dilution

1:330   1:500  

PROTEIN ARRAY

 
 

Antibody HPA055753

 

Antibody CAB002214

 

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
   

   

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

1:2650    

ANTIGEN INFORMATION

 
 

Antibody HPA055753

 

Antibody CAB002214

 

Antigen

Recombinant protein fragment   Not known  

Length (aa)

46    

Antigen sequence

PGPGIDMSAFAGLGPREKGPDPLQYMRADQAAGGLRQHDAEVDATL
 
 

Matching transcripts

COL2A1-001 - ENSP00000369889 [100%]
COL2A1-008 - ENSP00000338213 [100%]
   

Other gene match

   
ANTIGEN VIEW
COL2A1-001
COL2A1-008
 

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