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Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.
Antibody dilution
1:66
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA055753
Antibody CAB002214
Standard validation
Supported
Figure description
Immunohistochemical staining of human nasopharynx shows moderate cytoplasmic positivity in chondrocytes.
Expression
RNA: detected in 4 tissues Protein: detected in 11 cell types
Retrieval
None
Antibody dilution
1:50
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA055753
Antibody CAB002214
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
141.8, 134.4
141.8, 134.4
Antibody dilution
1:330
1:500
PROTEIN ARRAY
Antibody HPA055753
Antibody CAB002214
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
