Fields »
ABOUT
HELP
BLOG
RB1

ANTIBODY INFORMATION
 
 

Antibody HPA050082

  

Antibody CAB000095

  

Antibody CAB016687

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Pharmingen
   AbFrontier
  

Product name

 HPA050082   554136   LF-MA0173  

Host species

 Rabbit   Mouse   Mouse  

Clonality

 pAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known   Ammonium Sulfate Precipitation  

Other gene match

      

Released in version

 14   1   5  

References

      

Proper citation

 Atlas Antibodies Cat#HPA050082, RRID:AB_2681008     ABFrontier Cat#LF-MA0173, RRID:AB_1622010  

VALIDATION SUMMARY
 
 

Antibody HPA050082

  

Antibody CAB000095

  

Antibody CAB016687

  
Immunocytochemistry

  Supported   Supported  
Immunohistochemistry

Supported     Supported  
Western blot

Uncertain   Supported   Supported  
Protein array

Supported      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA050082

  

Antibody CAB000095

  

Antibody CAB016687

  

Standard validation

   Supported   Supported  

     

Figure description

   Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.   Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.  

Antibody dilution

   1:75   1:31  

Literature conformity

   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA050082

  

Antibody CAB000095

  

Antibody CAB016687

  

Standard validation

 Supported     Supported  

     

Figure description

 Immunohistochemical staining of retina shows strong nuclear positivity in photoreceptor cells, cells in inner nuclear layer and ganglion cells.     Immunohistochemical staining of retina shows moderate nuclear positivity in photoreceptor cells, cells in inner nuclear layer and ganglion cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 67 cell types		     RNA: detected in 37 tissues
Protein: detected in 53 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:800     1:125  

Literature conformity

 Consistent with extensive gene/protein characterization data.     Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.     Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA050082

  

Antibody CAB000095

  

Antibody CAB016687

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 106.2   106.2   106.2  

Antibody dilution

 1:190   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA050082

  

Antibody CAB000095

  

Antibody CAB016687

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:1500      

ANTIGEN INFORMATION
 
 

Antibody HPA050082

  

Antibody CAB000095

  

Antibody CAB016687

  

Antigen

 Recombinant protein fragment   Not known   Recombinant protein  

Length (aa)

 116      

Antigen sequence

 
PLVRLEFEETEEPDFTALCQKLKIPDHVRERAWLTWEKVSSVDGVLGGYI
QKKKELWGICIFIAAVDLDEMSFTFTELQKNIEISVHKFFNLLKEIDTST
KVDNAMSRLLKKYDVL
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					RB1-002 - ENSP00000267163 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
RB1-002
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

					Contact
				

				
				

					
					
					
					
				

				
			


			

				

					The Project
				
			
				
			

			
			

			
				

					The Human Protein Atlas
				

				
			

			
			

			

						The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.