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Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
Antibody dilution
1:80
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA001297
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 36 tissues Protein: detected in 49 cell types
Retrieval
HIER pH6
Antibody dilution
1:500
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Standard validation
Approved
Figure description
Cytoplasmic and nuclear positivity in neurons in most areas of the brain, most abundant in olfactory bulb, selected areas of the cerebral cortex as well as ventricle wall choroid plexus.
WESTERN BLOT
Antibody HPA001297
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5 Lane 2: RT4 Lane 3: EFO-21 Lane 4: A-431 Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
72, 71, 62.9, 29.2
Antibody dilution
1:500
PROTEIN ARRAY
Antibody HPA001297
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
