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Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, nuclear bodies, plasma membrane & cytosol.
Antibody dilution
1:106
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA042052
Antibody HPA061117
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human pancreas shows moderate membranous positivity in exocrine cells.
Immunohistochemical staining of human small intestine shows strong cytoplasmic and membranous positivity in glandular cells.
Expression
RNA: detected in 35 tissues Protein: detected in 30 cell types
RNA: detected in 35 tissues Protein: detected in 45 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:1000
1:450
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA042052
Antibody HPA061117
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY406295)
Target mass (kDa)
35.7
35.7, 23.9
Antibody dilution
1:250
1:180
PROTEIN ARRAY
Antibody HPA042052
Antibody HPA061117
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
