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Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & plasma membrane.
Antibody dilution
1:200
Literature conformity
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA042370
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human urinary bladder shows moderate nuclear positivity in urothelial cells.
Expression
RNA: detected in 37 tissues Protein: detected in 66 cell types
Retrieval
HIER pH6
Antibody dilution
1:3500
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA042370
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401800)
Target mass (kDa)
52
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA042370
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
