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Antibody staining overlaps with antibody HPA024089. Antibody staining overlaps with antibody HPA018993.
Validated
Antibody staining overlaps with antibody HPA024089. Antibody staining overlaps with antibody HPA018990.
Validated
Antibody staining overlaps with antibody HPA018993. Antibody staining overlaps with antibody HPA018990.
Standard validation
Supported
Supported
Supported
Figure description
Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
Antibody dilution
1:25
1:49
1:100
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA018990
Antibody HPA018993
Antibody HPA018996
Antibody HPA024089
Formal validation: Independent
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA024089 across 45 cell lines. Pearson correlation with HPA018996 across 45 cell lines. Pearson correlation with HPA018993 across 45 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA018996 across 45 cell lines. Pearson correlation with HPA018990 across 45 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA024089 across 45 cell lines. Pearson correlation with HPA018993 across 45 cell lines. Pearson correlation with HPA018990 across 45 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA018996 across 45 cell lines. Pearson correlation with HPA018990 across 45 cell lines.
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA024089 across 74 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA024089 across 72 cell types. Spearman correlation with HPA018996 across 73 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA024089 across 73 cell types. Spearman correlation with HPA018993 across 73 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA018996 across 73 cell types. Spearman correlation with HPA018993 across 72 cell types. Spearman correlation with HPA018990 across 74 cell types.
Standard validation
Supported
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human colon shows strong cytoplasmic positivity with a granular pattern, in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in granular pattern in glandular cells.
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in granular pattern in glandular cells.
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 77 cell types
RNA: detected in 37 tissues Protein: detected in 41 cell types
RNA: detected in 37 tissues Protein: detected in 25 cell types
RNA: detected in 37 tissues Protein: detected in 67 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:150
1:125
1:15
1:800
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA018990
Antibody HPA018993
Antibody HPA018996
Antibody HPA024089
Formal validation: Genetic
Validated
Downregulation visible in both siRNA lanes
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: siRNA 1 Lane 3: siRNA 2 Lane 4: Scrambled
