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IGF1R

ANTIBODY INFORMATION
 
 

Antibody HPA045563

  

Antibody CAB010268

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA045563   sc-713  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 13   4  

References

    

Proper citation

 Atlas Antibodies Cat#HPA045563, RRID:AB_2679368   Santa Cruz Biotechnology Cat#sc-713, RRID:AB_671792  

VALIDATION SUMMARY
 
 

Antibody HPA045563

  

Antibody CAB010268

  
Immunocytochemistry

  Approved  
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA045563

  

Antibody CAB010268

  

Standard validation

   Approved  

   

Figure description

   Immunofluorescent staining of human cell line A-431 shows localization to vesicles.  

Antibody dilution

   1:40  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA045563

  

Antibody CAB010268

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.   Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells of tubules.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 41 cell types		   RNA: detected in 36 tissues
Protein: detected in 69 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:500   1:50  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA045563

  

Antibody CAB010268

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

    

Target mass (kDa)

 154.8   154.8, 24.2, 21.3, 11.1  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA045563

  

Antibody CAB010268

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:6000    

ANTIGEN INFORMATION
 
 

Antibody HPA045563

  

Antibody CAB010268

  

Antigen

 Recombinant protein fragment   Synthetic peptide  

Length (aa)

 73    

Antigen sequence

 
YIVRWQRQPQDGYLYRHNYCSKDKIPIRKYADGTIDIEEVTENPKTEVCG
GEKGPCCACPKTEAEKQAEKEEA
  

											 
																				

																		

										
											
Matching transcripts

												
																					IGF1R-001 - ENSP00000268035 [100%] 
IGF1R-002 - ENSP00000453007 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
IGF1R-001
IGF1R-002
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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